对已建立的蚊虫细胞系的核型研究。
Karyological studies on established mosquito cell lines.
作者信息
Chiplonkar J M, Wagh U V, Phondke G P, Joshi D S
出版信息
Experientia. 1983 Jun 15;39(6):608-10. doi: 10.1007/BF01971121.
PMID:6852197
Abstract
Chromosome frequency distribution and cellular DNA estimations in different established mosquito cell lines were studied. These cell lines exhibited a wide range of cell types with a diploid stem-line comprising 50-55% and a haploid substem-line comprising 12-30% of the population. Estimation of cellular DNA contents by impulse cytoflowmetry and by Feulgen cytophotometry supported these observations. Because of their low diploid counts, these cell lines cannot be classified as diploid.
摘要
研究了不同已建立的蚊细胞系中的染色体频率分布和细胞DNA估计值。这些细胞系表现出广泛的细胞类型，其中二倍体主干系占群体的50-55%，单倍体亚主干系占12-30%。通过脉冲细胞流式仪和福尔根细胞光度法对细胞DNA含量的估计支持了这些观察结果。由于其二倍体计数较低，这些细胞系不能归类为二倍体。
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