一项关于兔肌肉肌酸激酶的反应性巯基与发色报告基团作用的研究。
A study of the role of the reactive thiol group of rabbit muscle creatine kinase with a chromophoric reporter group.
作者信息
Keighren M A, Price N C
出版信息
Biochem J. 1978 Apr 1;171(1):269-72. doi: 10.1042/bj1710269.
Abstract
Substrate- and ligand-induced conformational changes were studied in a series of thiol-modified derivatives of rabbit muscle creatine kinase that retained different amounts of enzymic activity. The results indicate that the 'reactive' thiol group of the enzyme is required for the conformational changes associated with formation of a 'transition-state analogue' complex.
摘要
在一系列保留不同酶活性水平的兔肌肉肌酸激酶硫醇修饰衍生物中，研究了底物和配体诱导的构象变化。结果表明，与“过渡态类似物”复合物形成相关的构象变化需要酶的“活性”硫醇基团。
相似文献
1
A study of the role of the reactive thiol group of rabbit muscle creatine kinase with a chromophoric reporter group.一项关于兔肌肉肌酸激酶的反应性巯基与发色报告基团作用的研究。
Biochem J. 1978 Apr 1;171(1):269-72. doi: 10.1042/bj1710269.
2
The effect of limited proteolysis on rabbit muscle creatine kinase.有限蛋白水解对兔肌肉肌酸激酶的影响。
Biochem J. 1981 Oct 1;199(1):239-44. doi: 10.1042/bj1990239.
3
The reaction of rabbit muscle creatine kinase with diethyl pyrocarbonate.兔肌肉肌酸激酶与焦碳酸二乙酯的反应
Biochem J. 1979 Aug 1;181(2):467-75. doi: 10.1042/bj1810467.
4
The nature and reactivity of the "essential" thiol in rabbit muscle creatine kinase III (EC 2.7.3.2).
Biochem Biophys Res Commun. 1982 Jul 16;107(1):302-6. doi: 10.1016/0006-291x(82)91704-1.
5
The reaction of rabbit muscle creatine kinase with some derivatives of iodoacetamide.兔肌酸激酶与碘乙酰胺某些衍生物的反应。
Biochem J. 1979 Feb 1;177(2):603-12. doi: 10.1042/bj1770603.
6
The refolding of denatured rabbit muscle creatine kinase. Search for intermediates in the refolding process and effect of modification at the reactive thiol group on refolding.变性兔肌肉肌酸激酶的重折叠。探寻重折叠过程中的中间体以及活性巯基修饰对重折叠的影响。
Biochem J. 1982 Jan 1;201(1):171-7. doi: 10.1042/bj2010171.
7
Non-identical behaviour of the subunits of rabbit muscule creatine kinase.兔肌肉肌酸激酶亚基的不同行为
Biochim Biophys Acta. 1976 Sep 14;445(2):364-76. doi: 10.1016/0005-2744(76)90090-5.
8
The tyrosyl residues in creatine kinase. Modification by iodine.
Biochim Biophys Acta. 1975 Oct 20;405(2):324-39. doi: 10.1016/0005-2795(75)90098-7.
9
Reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified creatine kinase reactivated by dithiothreitol.用二硫苏糖醇重新激活5,5'-二硫代双-(2-硝基苯甲酸)修饰的肌酸激酶的重新激活动力学
Biochim Biophys Acta. 1998 Oct 14;1388(1):190-8. doi: 10.1016/s0167-4838(98)00194-0.
10
Creatine kinase is modified by 2-chloromercuri-4-nitrophenol at the active site thiols with complete inactivation.肌酸激酶在活性位点硫醇处被2-氯汞基-4-硝基苯酚修饰，导致完全失活。
Biochim Biophys Acta. 1989 Jul 27;997(1-2):78-82. doi: 10.1016/0167-4838(89)90137-4.
引用本文的文献
1
The effect of limited proteolysis on rabbit muscle creatine kinase.有限蛋白水解对兔肌肉肌酸激酶的影响。
Biochem J. 1981 Oct 1;199(1):239-44. doi: 10.1042/bj1990239.
2
The reaction of rabbit muscle creatine kinase with diethyl pyrocarbonate.兔肌肉肌酸激酶与焦碳酸二乙酯的反应
Biochem J. 1979 Aug 1;181(2):467-75. doi: 10.1042/bj1810467.
3
The reaction of rabbit muscle creatine kinase with some derivatives of iodoacetamide.兔肌酸激酶与碘乙酰胺某些衍生物的反应。
Biochem J. 1979 Feb 1;177(2):603-12. doi: 10.1042/bj1770603.
本文引用的文献
1
Inhibition of adenosine 5'-triphosphate-creatine phosphotransferase by substrate-anion complexes. Evidence for the transition-state organization of the catalytic site.底物 - 阴离子复合物对腺苷5'-三磷酸 - 肌酸磷酸转移酶的抑制作用。催化位点过渡态组织的证据。
Biochem J. 1971 May;122(5):727-40. doi: 10.1042/bj1220727.
2
The role of the lysyl residue at the active site of creatine kinase. Nuclear Overhauser effect studies.
J Biol Chem. 1974 Apr 25;249(8):2599-604.
3
The interaction of 8-anilino-1-naphthalenesulfonate with creatine kinase. Evidence for cooperativitiy of nucleotide binding.8-苯胺基-1-萘磺酸盐与肌酸激酶的相互作用。核苷酸结合协同性的证据。
J Biol Chem. 1974 Mar 10;249(5):1445-52.
4
Substrate binding to an active creatine kinase with a thiol-bound mercurinitrophenol chromophoric probe.底物与带有硫醇结合的汞硝基苯酚发色探针的活性肌酸激酶的结合。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2858-62. doi: 10.1073/pnas.70.10.2858.
5
Conformational flexibility and structure of creatine kinase.肌酸激酶的构象灵活性与结构
J Supramol Struct. 1975;3(2):192-9. doi: 10.1002/jss.400030213.
6
Preparation and properties of S-cyano derivatives of creatine kinase.
Eur J Biochem. 1976 Nov 15;70(2):623-8. doi: 10.1111/j.1432-1033.1976.tb11053.x.
7
Creatine kinase. Modification of the working enzyme.肌酸激酶。活性酶的修饰。
Biochem J. 1976 Jul 1;157(1):23-31. doi: 10.1042/bj1570023.
8
Non-identical behaviour of the subunits of rabbit muscule creatine kinase.兔肌肉肌酸激酶亚基的不同行为
Biochim Biophys Acta. 1976 Sep 14;445(2):364-76. doi: 10.1016/0005-2744(76)90090-5.
9
Properties of a CH3-blocked creatine kinase with altered catalytic activity. Kinetic consequences of the presence of the blocking group.
J Biol Chem. 1977 Feb 25;252(4):1202-7.
Zotero 插件