有限蛋白水解对兔肌肉肌酸激酶的影响。
The effect of limited proteolysis on rabbit muscle creatine kinase.
作者信息
Price N C, Murray S, Milner-White E J
出版信息
Biochem J. 1981 Oct 1;199(1):239-44. doi: 10.1042/bj1990239.
Abstract
Creatine kinase from rabbit muscle is inactivated by limited proteolysis with proteinase K from Tritirachium album. Gel-filtration and cross-linking studies showed that the limited proteolysis did not affect the molecular weight of the enzyme under non-denaturing conditions, but did cause changes in the reactivity of the reactive thiol group on each subunit and in the ability of the enzyme to form a 'transition-state analogue' complex in the presence of magnesium acetate plus ADP plus creatinine plus NaNO3.
摘要
兔肌肉中的肌酸激酶会被来自特异腐质霉的蛋白酶K进行有限的蛋白水解作用而失活。凝胶过滤和交联研究表明，在非变性条件下，有限的蛋白水解作用不会影响该酶的分子量，但确实会导致每个亚基上反应性巯基的反应性发生变化，以及在乙酸镁、ADP、肌酸酐和硝酸钠存在的情况下，该酶形成“过渡态类似物”复合物的能力发生变化。
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