一种适用于小多肽的简单灵敏方法，用于在等电聚焦后测定聚丙烯酰胺凝胶中的蛋白质。
A simple sensitive method, applicable to small polypeptides, for the determination of proteins in polyacrylamide gels after isoelectric focusing.
作者信息
Storring P L, Tiplady R J
出版信息
Biochem J. 1978 Apr 1;171(1):79-82. doi: 10.1042/bj1710079.
Abstract
A simple method is described for the determination of polypeptides and proteins in polyacrylamide gels after isoelectric focusing. Precipitates formed in trichloroacetic acid, under controlled conditions, are quantified densitometrically by measuring the proportion of light scattered. The procedure is of particular value in its applicability to smaller polypeptides, with mol.wts. of 3000-6000, which are not fixed adequately in gels by other procedures currently in use. The working range, over which polypeptide concentration is proportional to the effective absorbance, is approx. 1-30 microgram per component.
摘要
本文描述了一种简单的方法，用于在等电聚焦后测定聚丙烯酰胺凝胶中的多肽和蛋白质。在受控条件下，三氯乙酸中形成的沉淀通过测量散射光的比例进行光密度定量。该方法特别适用于分子量为3000-6000的较小多肽，而目前使用的其他方法无法在凝胶中充分固定这些多肽。多肽浓度与有效吸光度成正比的工作范围约为每个组分1-30微克。
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