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组蛋白在聚丙烯酰胺凝胶中的电泳及其定量测定。

The electrophoresis of histones in polyacrylamide gel and their quantitative determination.

作者信息

Johns E W

出版信息

Biochem J. 1967 Jul;104(1):78-82. doi: 10.1042/bj1040078.

DOI:10.1042/bj1040078
PMID:6035526
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1270547/
Abstract
  1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel.
摘要
  1. 已设计出一种新方法，用于在pH2.4的聚丙烯酰胺凝胶中通过电泳分离小牛胸腺组蛋白组分。2. 这些组分已根据它们相对于标记蛋白牛血清白蛋白的相对迁移率进行了表征。3. 已开发出一种方法，通过测量凝胶中形成的染料-组蛋白复合物的颜色产量来定量测定分离出的组蛋白组分。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad59/1270547/77fa34ec3b93/biochemj00741-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad59/1270547/f41d340d1c03/biochemj00741-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad59/1270547/77fa34ec3b93/biochemj00741-0091-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad59/1270547/f41d340d1c03/biochemj00741-0091-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad59/1270547/77fa34ec3b93/biochemj00741-0091-b.jpg

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[Study of the heterogeneity of calf thymus histones by polyacrylamide gel electrophoresis].[通过聚丙烯酰胺凝胶电泳对小牛胸腺组蛋白异质性的研究]
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本文引用的文献

1
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
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On the complexity of calf thymus histone.论小牛胸腺组蛋白的复杂性。
Biochemistry. 1962 Jan;1:79-89. doi: 10.1021/bi00907a013.
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METABOLISM OF HISTONES IN MALIGNANT TISSUES AND LIVER OF THE RAT AND MOUSE.大鼠和小鼠恶性组织及肝脏中组蛋白的代谢
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Histones H1 and H5: one or two molecules per nucleosome?组蛋白H1和H5：每个核小体有一个还是两个分子？
Nucleic Acids Res. 1981 Nov 25;9(22):5883-94. doi: 10.1093/nar/9.22.5883.
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Regulation of the higher-order structure of chromatin by histones H1 and H5.组蛋白H1和H5对染色质高级结构的调控。
J Cell Biol. 1981 Aug;90(2):279-88. doi: 10.1083/jcb.90.2.279.
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Specific interaction of histone H1 with eukaryotic DNA.组蛋白H1与真核生物DNA的特异性相互作用。
Nucleic Acids Res. 1981 Mar 25;9(6):1383-93. doi: 10.1093/nar/9.6.1383.
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A polyacrylamide-gel electrophoretic study of human tear proteins.
Graefes Arch Clin Exp Ophthalmol. 1982;219(2):95-9. doi: 10.1007/BF02173448.
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Correlation between endogenous nucleosomal hyper(ADP-ribosyl)ation of histone H1 and the induction of chromatin relaxation.组蛋白H1的内源性核小体超（ADP-核糖基）化与染色质松弛诱导之间的相关性。
EMBO J. 1983;2(10):1685-93. doi: 10.1002/j.1460-2075.1983.tb01643.x.
9
Accumulation of the isolated carboxy-terminal domain of histone H1 in the Xenopus oocyte nucleus.组蛋白H1分离的羧基末端结构域在非洲爪蟾卵母细胞核中的积累。
EMBO J. 1984 Sep;3(9):1933-7. doi: 10.1002/j.1460-2075.1984.tb02072.x.
10
The histones of some human tissues.一些人体组织的组蛋白。
Biochem J. 1968 Nov;110(2):181-5. doi: 10.1042/bj1100181.
Biochem J. 1965 Jul;96(1):53-62. doi: 10.1042/bj0960053.
4
A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS.小牛胸腺F2A组组蛋白的分级分离
Biochem J. 1965 Jan;94(1):127-30. doi: 10.1042/bj0940127.
5
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
6
HISTONES: SPECIES AND TISSUE SPECIFICITY.组蛋白：物种及组织特异性
Science. 1964 Sep 4;145(3636):1059-61. doi: 10.1126/science.145.3636.1059.
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BASIC AND OTHER PROTEINS IN MICROSOMES OF RAT LIVER.大鼠肝脏微粒体中的碱性蛋白及其他蛋白
Biochem J. 1963 Aug;88(2):206-12. doi: 10.1042/bj0880206.
8
Fractionation of the histones of the Walker 256 carcinosarcoma by combined chemical and chromatographic techniques.运用化学和色谱技术相结合的方法对沃克256癌肉瘤的组蛋白进行分级分离。
J Biol Chem. 1963 Mar;238:918-24.
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The presence of acetyl groups of histones.组蛋白乙酰基的存在。
Biochem J. 1963 May;87(2):258-63. doi: 10.1042/bj0870258.
10
Observations on the species and tissue specificity of histones.关于组蛋白的物种和组织特异性的观察
Biochem J. 1962 Jan;82(1):123-9. doi: 10.1042/bj0820123.