组蛋白在聚丙烯酰胺凝胶中的电泳及其定量测定。
The electrophoresis of histones in polyacrylamide gel and their quantitative determination.
作者信息
Johns E W
出版信息
Biochem J. 1967 Jul;104(1):78-82. doi: 10.1042/bj1040078.
Abstract
- A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel.
摘要
- 已设计出一种新方法，用于在pH2.4的聚丙烯酰胺凝胶中通过电泳分离小牛胸腺组蛋白组分。2. 这些组分已根据它们相对于标记蛋白牛血清白蛋白的相对迁移率进行了表征。3. 已开发出一种方法，通过测量凝胶中形成的染料-组蛋白复合物的颜色产量来定量测定分离出的组蛋白组分。
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