[大鼠肝脏5S核糖体RNA的构象异构体]

[Conformational isomers of rat liver 5S ribosomal RNA].

作者信息

Kel've M B, Metspalu A Kh, Lind A Ia, Saarma M Iu, Villems R L

出版信息

Mol Biol (Mosk). 1978 May-Jun;12(3):695-9.

PMID:96331

Abstract

The rat liver 5S RNA when denaturated by urea or EDTA, or even without any special treatment, undergoes conformational changes leading to the formation of three electrophoretically distinct isomeres of the molecules with relative mobilities 0.39, 0.44 and 0.47. The band with the slowest mobility corresponds apparently to the native 5S RNA since it is specific for both freshy isolated and renaturated 5S RNA. Moreover, it was found that denaturation of the immobilized 5 S RNA decreases significantly its ability to form a complex with the rat liver 60S ribosomal subunit proteins L6, L7, L8, L18 and L35.

摘要

大鼠肝脏5S RNA在被尿素或乙二胺四乙酸（EDTA）变性时，甚至在没有任何特殊处理的情况下，会发生构象变化，导致形成三种电泳性质不同的分子异构体，其相对迁移率分别为0.39、0.44和0.47。迁移率最慢的条带显然对应于天然5S RNA，因为它对新鲜分离的和复性的5S RNA都是特异性的。此外，还发现固定化的5S RNA变性后，其与大鼠肝脏60S核糖体亚基蛋白L6、L7、L8、L18和L35形成复合物的能力显著降低。

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引用本文的文献

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Two distinct conformations of rat liver ribosomal 5S RNA.大鼠肝脏核糖体5S RNA的两种不同构象。

Nucleic Acids Res. 1982 Jun 11;10(11):3381-9. doi: 10.1093/nar/10.11.3381.

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The secondary structure of oocyte and somatic 5S ribosomal RNAs of the fish Misgurnus fossilis L. from nuclease hydrolyses and chemical modification data.基于核酸酶水解和化学修饰数据对泥鳅（Misgurnus fossilis L.）卵母细胞和体细胞5S核糖体RNA二级结构的研究

Nucleic Acids Res. 1984 Jul 11;12(13):5385-404. doi: 10.1093/nar/12.13.5385.

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Location of single-stranded and double-stranded regions in rat liver ribosomal 5S RNA and 5.8S RNA.大鼠肝脏核糖体5S RNA和5.8S RNA中单链和双链区域的定位

Nucleic Acids Res. 1981 Oct 24;9(20):5331-43. doi: 10.1093/nar/9.20.5331.

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