Johnson M C, Beebe D C
Exp Eye Res. 1984 Jun;38(6):579-92. doi: 10.1016/0014-4835(84)90176-3.
The growth, synthesis and regional specialization of the lens capsule has been studied in chicken embryos and compared to adult chickens and mammals. During the final 15 days of embryonic development the surface area of the capsule increased 11-fold. This represents the minimum estimate of capsule growth, since it also increased in thickness during this period. Autoradiographic examination of [3H]proline- or 35SO4-labeled, detergent-cleaned, flat-mounted capsules suggested that all lens cells participated in capsule synthesis. This was supported by the observation that both lens epithelial and fiber cells incorporated [35S]-methionine into collagenase-sensitive proteins with molecular weights similar to type IV collagen. Histochemical staining of detergent-cleaned capsule explants (DCCEs) revealed regional differences in the carbohydrate composition of the capsule. Differences in collagenous proteins were also seen between the anterior and posterior regions of the capsule of the embryonic lenses. Following SDS-PAGE and silver staining a total of six collagenase-sensitive proteins were seen with molecular weights between 150 and 180 K. Three of these polypeptides were common to both anterior and posterior capsules, one was found only in the anterior capsule and the other two were found predominantly in the posterior capsule. No regional differences were seen in the collagenous proteins of capsules from adult chickens, rats or hamsters. The possibility is discussed that the unique pattern of collagenous polypeptides seen in embryonic capsules is related to the rapid growth of the embryonic lens.
已对鸡胚晶状体囊的生长、合成及区域特化进行了研究，并与成年鸡和哺乳动物进行了比较。在胚胎发育的最后15天里，晶状体囊的表面积增加了11倍。这只是晶状体囊生长的最低估计值，因为在此期间其厚度也有所增加。对用[³H]脯氨酸或³⁵SO₄标记、经去污剂处理并平铺的晶状体囊进行放射自显影检查表明，所有晶状体细胞都参与了晶状体囊的合成。晶状体上皮细胞和纤维细胞都将[³⁵S] - 甲硫氨酸掺入分子量与IV型胶原相似的对胶原酶敏感的蛋白质中，这一观察结果支持了上述结论。对经去污剂处理的晶状体囊外植体（DCCEs）进行组织化学染色，揭示了晶状体囊碳水化合物组成的区域差异。在胚胎晶状体囊的前部和后部区域之间，也观察到了胶原蛋白质的差异。经过SDS - PAGE和银染后，共发现了六种对胶原酶敏感的蛋白质，分子量在150至180K之间。其中三种多肽在前部和后部晶状体囊中都有，一种仅在前部晶状体囊中发现，另外两种主要在后部晶状体囊中发现。在成年鸡、大鼠或仓鼠的晶状体囊中，未观察到胶原蛋白质的区域差异。文中讨论了胚胎晶状体囊中所见胶原多肽独特模式与胚胎晶状体快速生长相关的可能性。
