Wakely J
Exp Eye Res. 1984 Jun;38(6):627-36. doi: 10.1016/0014-4835(84)90181-7.
The importance of differential epithelial spreading in the early stages of chick lens placode invagination in the chick has been demonstrated experimentally in two ways. Generalized swelling of the head caused by immersion in 50% ethanol and restricting the freedom of ectoderm in the eye region to spread by means of ligatures produce both precocious and exaggerated invagination. Inequality of spreading between the placode and surrounding ectoderm in normal development is caused by the adhesion of the former to the optic vesicle, while the ectoderm is free to spread and move over underlying tissues. Folds, i.e. invaginations, form at the boundary between the placode and its surroundings.
在雏鸡中，通过两种实验方法证明了差异上皮扩展在雏鸡晶状体基板内陷早期阶段的重要性。将头部浸入50%乙醇中导致的全身性肿胀，以及通过结扎限制眼区外胚层的扩展自由，都会产生早熟和过度的内陷。在正常发育过程中，基板与周围外胚层之间扩展的不平等是由前者与视泡的粘连引起的，而外胚层则可以自由地在下方组织上扩展和移动。褶皱，即内陷，在基板与其周围环境的边界处形成。
