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人体组织中多胺氧化酶活性的测定

Determination of polyamine oxidase activities in human tissues.

作者信息

Suzuki O, Matsumoto T, Katsumata Y

出版信息

Experientia. 1984 Aug 15;40(8):838-9. doi: 10.1007/BF01951981.

DOI:10.1007/BF01951981
PMID:6468593
Abstract

A very simple fluorometric assay for polyamine oxidase (PAO) in tissues, with N1-monoacetylspermine as substrate, is described. The PAO was present in all human organs tested; it was highest in the liver, followed by the testis, kidney, spleen and small intestine.

摘要

本文描述了一种以N1-单乙酰精胺为底物,用于检测组织中多胺氧化酶(PAO)的非常简单的荧光测定法。在所检测的所有人体器官中均存在PAO;其在肝脏中含量最高,其次是睾丸、肾脏、脾脏和小肠。

相似文献

1
Determination of polyamine oxidase activities in human tissues.人体组织中多胺氧化酶活性的测定
Experientia. 1984 Aug 15;40(8):838-9. doi: 10.1007/BF01951981.
2
Occurrence of polyamine oxidase in tissues of the catfish.鲶鱼组织中多胺氧化酶的存在情况。
Comp Biochem Physiol B. 1987;88(3):727-8. doi: 10.1016/0305-0491(87)90235-5.
3
Distribution of polyamine oxidase activity in rat tissues and subcellular fractions.大鼠组织和亚细胞组分中多胺氧化酶活性的分布
Experientia. 1991 Dec 1;47(11-12):1209-11. doi: 10.1007/BF01918386.
4
In situ substrate specificity and ultrastructural localization of polyamine oxidase activity in unfixed rat tissues.未固定大鼠组织中多胺氧化酶活性的原位底物特异性及超微结构定位
J Histochem Cytochem. 1995 Nov;43(11):1155-62. doi: 10.1177/43.11.7560898.
5
Metabolism of acetylpolyamines by monoamine oxidase, diamine oxidase and polyamine oxidase.单胺氧化酶、二胺氧化酶和多胺氧化酶对乙酰多胺的代谢作用。
Biochim Biophys Acta. 1981 Oct 12;677(2):190-3. doi: 10.1016/0304-4165(81)90084-2.
6
Androgenic sensitivity of polyamine-oxidizing enzyme activities in female rat tissues.雌性大鼠组织中多胺氧化酶活性的雄激素敏感性
Amino Acids. 2000;18(4):399-405. doi: 10.1007/s007260070078.
7
Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion.参与多胺逆向转化的哺乳动物多胺氧化酶的基因组鉴定及生化特性分析
Biochem J. 2003 Feb 15;370(Pt 1):19-28. doi: 10.1042/BJ20021779.
8
A conceptual model of the polyamine binding site of N1-acetylpolyamine oxidase developed from a study of polyamine derivatives.基于对多胺衍生物的研究建立的N1-乙酰多胺氧化酶多胺结合位点的概念模型。
Amino Acids. 2009 Jul;37(2):401-5. doi: 10.1007/s00726-008-0168-9. Epub 2008 Aug 19.
9
Polyamine oxidase in rat tissues.大鼠组织中的多胺氧化酶。
Biochim Biophys Acta. 1980 Oct;615(2):480-8. doi: 10.1016/0005-2744(80)90514-8.
10
Polyamine oxidase activity and concentration of polyamines in tissues and serum of hamsters with transplantational pigmented melanoma IC-Sofia.移植性色素沉着性黑色素瘤IC-Sofia仓鼠组织和血清中的多胺氧化酶活性及多胺浓度
Acta Physiol Pharmacol Bulg. 1991;17(4):53-8.

引用本文的文献

1
Controlling the regioselectivity and stereospecificity of FAD-dependent polyamine oxidases with the use of amine-attached guide molecules as conformational modulators.利用连接胺的导向分子作为构象调节剂控制 FAD 依赖性多胺氧化酶的区域选择性和立体特异性。
Biosci Rep. 2018 Aug 29;38(4). doi: 10.1042/BSR20180527. Print 2018 Aug 31.
2
Metabolism of N-alkylated spermine analogues by polyamine and spermine oxidases.多胺氧化酶和精脒氧化酶对 N-烷基化精脒类似物的代谢。
Amino Acids. 2010 Feb;38(2):369-81. doi: 10.1007/s00726-009-0429-2. Epub 2009 Dec 10.
3
Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages.

本文引用的文献

1
Polyamine oxidase in rat tissues.大鼠组织中的多胺氧化酶。
Biochim Biophys Acta. 1980 Oct;615(2):480-8. doi: 10.1016/0005-2744(80)90514-8.
2
Acetylderivatives as intermediates in polyamine catabolism.乙酰衍生物作为多胺分解代谢的中间体。
Int J Biochem. 1981;13(3):287-92. doi: 10.1016/0020-711x(81)90080-x.
3
Increased sensitivity of the fluorometric method of Snyder and Hendley for oxidase assays.用于氧化酶测定的斯奈德和亨德利荧光法的灵敏度提高。
肺孢子菌介导抗酶抑制剂的过表达,导致肺泡巨噬细胞中多胺水平升高和细胞凋亡。
J Biol Chem. 2009 Mar 20;284(12):8174-84. doi: 10.1074/jbc.M805787200. Epub 2009 Jan 20.
4
Factors affecting the relative importance of amine oxidases and monooxygenases in the in vivo metabolism of xenobiotic amines in humans.影响胺氧化酶和单加氧酶在人体内异源生物胺体内代谢中相对重要性的因素。
J Neural Transm (Vienna). 2007;114(6):787-91. doi: 10.1007/s00702-007-0689-5. Epub 2007 Mar 31.
5
Cloning and characterization of multiple human polyamine oxidase splice variants that code for isoenzymes with different biochemical characteristics.多种人类多胺氧化酶剪接变体的克隆与特性分析,这些变体编码具有不同生化特性的同工酶。
Biochem J. 2002 Dec 15;368(Pt 3):673-7. doi: 10.1042/BJ20021587.
6
Oxidation of polyamines and brain injury.多胺氧化与脑损伤。
Neurochem Res. 2000 Apr;25(4):471-90. doi: 10.1023/a:1007508008731.
7
Changes in polyamine catabolism in HL-60 human promyelogenous leukaemic cells in response to etoposide-induced apoptosis.依托泊苷诱导HL-60人早幼粒细胞白血病细胞凋亡过程中多胺分解代谢的变化。
Biochem J. 1999 Jan 1;337 ( Pt 1)(Pt 1):83-7.
8
Spermine inhibits proinflammatory cytokine synthesis in human mononuclear cells: a counterregulatory mechanism that restrains the immune response.精胺抑制人单核细胞中促炎细胞因子的合成:一种抑制免疫反应的反调节机制。
J Exp Med. 1997 May 19;185(10):1759-68. doi: 10.1084/jem.185.10.1759.
9
Distribution of polyamine oxidase activity in rat tissues and subcellular fractions.大鼠组织和亚细胞组分中多胺氧化酶活性的分布
Experientia. 1991 Dec 1;47(11-12):1209-11. doi: 10.1007/BF01918386.
10
Mechanisms of spermine toxicity in baby-hamster kidney (BHK) cells. The role of amine oxidases and oxidative stress.精胺对幼仓鼠肾(BHK)细胞的毒性机制。胺氧化酶和氧化应激的作用。
Biochem J. 1991 Nov 15;280 ( Pt 1)(Pt 1):193-8. doi: 10.1042/bj2800193.
Biochem Pharmacol. 1982 Jun 15;31(12):2207-9. doi: 10.1016/0006-2952(82)90520-2.
4
Metabolism of acetylpolyamines by monoamine oxidase, diamine oxidase and polyamine oxidase.单胺氧化酶、二胺氧化酶和多胺氧化酶对乙酰多胺的代谢作用。
Biochim Biophys Acta. 1981 Oct 12;677(2):190-3. doi: 10.1016/0304-4165(81)90084-2.
5
New substrates for the fluorometric determination of oxidative enzymes.用于荧光法测定氧化酶的新型底物。
Anal Chem. 1968 Jul;40(8):1256-63. doi: 10.1021/ac60264a027.
6
A simple and sensitive fluorescence assay for monoamine oxidase and diamine oxidase.一种用于单胺氧化酶和二胺氧化酶的简单灵敏的荧光测定法。
J Pharmacol Exp Ther. 1968 Oct;163(2):386-92.
7
Determination of the naturally occurring monoacetyl derivatives of di- and polyamines.二胺和多胺天然存在的单乙酰衍生物的测定。
J Chromatogr. 1979 Oct 11;164(2):155-68. doi: 10.1016/s0378-4347(00)81184-6.
8
Oxidation of spermidine and spermine in rat liver: purification and properties of polyamine oxidase.大鼠肝脏中精胺和亚精胺的氧化:多胺氧化酶的纯化及性质
Biochemistry. 1977 Jan 11;16(1):91-100. doi: 10.1021/bi00620a015.