Welter D A, Black D A, Hodge L D
Experientia. 1984 Aug 15;40(8):871-3. doi: 10.1007/BF01952002.
A new technique which removes all membranes, cytoskeletal elements, organelles, but preserves intact metaphase, anaphase and telophase configurations is combined with scanning electron microscopy (SEM) as an approach for direct visualization of chromosomal behavior in late mitosis. With this approach we are able to confirm the presence of a centromeric ring which stabilizes the centromeres during the cell cycle and present evidence for a lattice-like sheet of interchromatidic fibers in late mitosis.
一种新技术，它能去除所有膜、细胞骨架成分、细胞器，但保留完整的中期、后期和末期形态，该技术与扫描电子显微镜（SEM）相结合，作为直接观察有丝分裂后期染色体行为的一种方法。通过这种方法，我们能够证实存在一种着丝粒环，它在细胞周期中稳定着丝粒，并为有丝分裂后期染色单体间纤维形成的晶格状薄片提供证据。
