Korf B R, Diacumakos E G
Cytogenet Cell Genet. 1977;19(6):335-43. doi: 10.1159/000130827.
The positioning of metaphase chromosomes of the Indian muntjac (Muntiacus muntjak) in the radial metaphase array was investigated using two methods for examining cells. Large numbers of radial metaphase figures were obtained by mitotic shake-off. Treatment with hypotonic solution, fixation, and spreading onto glass slides did not disrupt the radial metaphase configuration of a proportion of the cells. Analysis of 567 cells for the arrangement of six of the seven muntjac chromosomes revealed a random positioning of each chromosomes within the radial array. These results were corroborated by results obtained from 101 living cells oriented with microneedles to enable examination of radial metaphases with phase-contrast microscopy.
采用两种细胞检测方法对印度麂（Muntiacus muntjak）中期染色体在径向中期排列中的定位进行了研究。通过有丝分裂振荡分离获得了大量的径向中期图像。用低渗溶液处理、固定并铺展在载玻片上，并未破坏一部分细胞的径向中期构型。对567个细胞中七条麂染色体中的六条的排列进行分析，结果显示每条染色体在径向排列中随机定位。通过用微针将101个活细胞定向，以便用相差显微镜检查径向中期，所得结果证实了上述结果。
