不育女性循环中免疫复合物的检测与特征分析。

Detection and characterization of immune complexes in the circulation of infertile women.

作者信息

Witkin S S, Bongiovanni A M, Berkeley A, Ledger W J, Toth A

出版信息

Fertil Steril. 1984 Sep;42(3):384-8. doi: 10.1016/s0015-0282(16)48077-5.

Abstract

The incidence of circulating immune complexes (CICs) was evaluated in sera from 39 female partners of infertile marriages and from 38 fertile women. Fifteen (38%) of the infertile women had CICs, as determined by the Raji cell assay, in levels ranging from 300 to 8000 micrograms/ml; whereas only 1 (3%) of the fertile women displayed CICs (P less than 0.001). Analysis of the CICs from nine of the women following polyethylene glycol precipitation and acid dissociation revealed that four contained C1q and three contained an antigen reactive with rabbit antibody to human spermatozoa. These latter three women all lacked free sperm antibody, as determined by enzyme-linked immunosorbent assay and agglutination. Thus, CICs are not uncommon as a manifestation of infertility in females. Their presence may lead to an underestimation of sperm antibody levels and may be indicative of underlying infection or autoimmunity.

摘要

对39名不育婚姻女性伴侣的血清以及38名可育女性的血清中的循环免疫复合物（CIC）发生率进行了评估。通过Raji细胞检测法测定，15名（38%）不育女性存在CIC，水平在300至8000微克/毫升之间；而可育女性中只有1名（3%）显示有CIC（P小于0.001）。对9名女性的CIC进行聚乙二醇沉淀和酸解离后分析发现，其中4个含有C1q，3个含有与兔抗人精子抗体反应的抗原。通过酶联免疫吸附测定法和凝集试验测定，后三名女性均缺乏游离精子抗体。因此，CIC作为女性不育的一种表现并不罕见。它们的存在可能导致精子抗体水平被低估，并且可能表明存在潜在感染或自身免疫。