Notermans S, Dufrenne J B
Antonie Van Leeuwenhoek. 1982 Dec;48(5):447-55. doi: 10.1007/BF00448416.
Staphyloccoccus aureus enterotoxin F (SEF), which is associated with S. aureus strains isolated from toxic-shock-syndrome patients, was purified by successive chromatography on CM sephadex C-25 and gelfiltration on sephadex G-75. When tested by disc-polyacrylamide gel-electrophoresis the toxin migrated as a homogeneous protein. In SDS-polyacrylamide gel-electrophoresis three protein bands were observed. The main component had a mol wt of 23 000 and the two minor components had a mol wt less than 13 000. By iso-electric focussing a main protein band with an iso-electric point of 7.2 was obtained. The LD50 for rabbits (3-3.5 kg) by subcutaneous and intravenous application of SEF was 6 micrograms and 180 micrograms, respectively. Antibodies to SEF prepared in a sheep did not react with other staphylococcal enterotoxins (A to E).
金黄色葡萄球菌肠毒素F（SEF）与从中毒性休克综合征患者分离出的金黄色葡萄球菌菌株相关，通过在CM葡聚糖凝胶C - 25上连续色谱分离和在葡聚糖凝胶G - 75上凝胶过滤进行纯化。当通过圆盘聚丙烯酰胺凝胶电泳测试时，该毒素以单一蛋白质形式迁移。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳中观察到三条蛋白带。主要成分的分子量为23000，两个次要成分的分子量小于13000。通过等电聚焦获得了一条等电点为7.2的主要蛋白带。通过皮下和静脉注射SEF，3 - 3.5千克兔子的半数致死量分别为6微克和180微克。在绵羊体内制备的抗SEF抗体不与其他葡萄球菌肠毒素（A至E）发生反应。
