Garver F A, Singh H, Moscoso H, McGuire B S, Kestler D P
Hemoglobin. 1984;8(2):105-15. doi: 10.3109/03630268408991704.
A highly specific method for the conclusive identification of normal and variant human hemoglobins is described in this communication. The method employs the standard electrophoretic technique in combination with the immunoblot technique using monospecific antisera raised in rabbits. Various hemoglobins such as Hb, S, C, A2, and F were separated on an alkaline polyacrylamide gel or a cellulose-acetate membrane, and transferred to a nitrocellulose membrane and the individual hemoglobins were identified by the immunoblot procedure. With this method hemoglobins with similar or identical electrophoretic mobilities can be definitively identified with the use of monospecific antisera.
本报告描述了一种用于最终鉴定正常和变异型人类血红蛋白的高度特异性方法。该方法采用标准电泳技术，并结合使用在兔体内产生的单特异性抗血清的免疫印迹技术。各种血红蛋白，如血红蛋白Hb、S、C、A2和F，在碱性聚丙烯酰胺凝胶或醋酸纤维素膜上分离，然后转移到硝酸纤维素膜上，并通过免疫印迹程序鉴定各个血红蛋白。使用这种方法，可以通过单特异性抗血清明确鉴定具有相似或相同电泳迁移率的血红蛋白。
