Varghese A J, Whitmore G F
Int J Radiat Oncol Biol Phys. 1984 Aug;10(8):1361-3. doi: 10.1016/0360-3016(84)90349-3.
Chemical studies have indicated that, following reduction of misonidazole to the hydroxylamine derivative, reaction with guanosine leads to the formation of a 2-carbon addition product of guanosine. In this study, the formation of the guanosine product is used to detect the presence of a reactive metabolite of misonidazole in the urine of patients treated with misonidazole. Urine samples were incubated with [14C]guanosine and the guanosine product was separated by HPLC analysis. The quantities of product vary as much as 10-fold from patient to patient and it is suggested that the assay might be useful as a predictor of patients susceptible to the development of peripheral neuropathy or other effects of misonidazole.
化学研究表明，米索硝唑还原为羟胺衍生物后，与鸟苷反应会生成鸟苷的一种2-碳加成产物。在本研究中，利用鸟苷产物的形成来检测接受米索硝唑治疗患者尿液中米索硝唑活性代谢物的存在。将尿液样本与[14C]鸟苷一起孵育，通过高效液相色谱分析分离鸟苷产物。不同患者的产物量相差可达10倍，提示该检测方法可能有助于预测易发生周围神经病变或米索硝唑其他效应的患者。
