Wetterau J R, Zilversmit D B
J Biol Chem. 1984 Sep 10;259(17):10863-6.
An intracellular protein accelerates the transfer of triglyceride and cholesteryl ester. The fraction of phospholipid transferred was much less than for the less polar lipids. A rich source of this activity was obtained from low ionic strength washes of liver microsomes. The protein was partially purified by column chromatography on Bio-Gel A-5m and hydroxylapatite. The elution position of the transfer protein on gel filtration corresponds to a protein with a molecular weight of about 200,000. The isoelectric point of the partially purified protein is between pH 5.2 and 5.6. At each step of the purification the stimulation of triglyceride transfer was greater than that of cholesteryl ester.
一种细胞内蛋白质可加速甘油三酯和胆固醇酯的转运。所转运的磷脂部分远少于极性较小的脂质。这种活性的丰富来源得自肝微粒体的低离子强度洗涤液。该蛋白质通过在Bio-Gel A-5m和羟磷灰石上进行柱色谱法进行部分纯化。在凝胶过滤中转运蛋白的洗脱位置对应于一种分子量约为200,000的蛋白质。部分纯化蛋白质的等电点在pH 5.2至5.6之间。在纯化的每个步骤中，甘油三酯转运的刺激作用都大于胆固醇酯。
