Adler L A, Arvidson S
J Gen Microbiol. 1984 Jul;130(7):1673-82. doi: 10.1099/00221287-130-7-1673.
Membrane ribosomes from Staphylococcus aureus which were detached from the membrane by extraction with the nonionic detergent Triton X-100 retained a protein (MBRP) with a molecular weight of 60 000, which was absent from cytoplasmic ribosomes. MBRP was detected and quantified by immunological methods. When membrane ribosomes were dissociated into 50S and 30S subunits, MBRP remained associated with the 50S particle. MBRP was found both on membrane ribosomes and in the cytoplasm in roughly equal amounts. When added to Triton X-100-solubilized protoplasts, antibodies to MBRP produced immunoprecipitates which contained a complex of MBRP and three other proteins with molecular weights of 71 000, 46 000 and 41 000. Four proteins with the same molecular weights as those of the MBRP complex were found associated with membrane ribosomes. The proteins of molecular weight 71 000, 60 000, 46 000 and 41 000 seemed to be present in stoichiometrically equivalent amounts in the complex.
用非离子去污剂 Triton X - 100 从金黄色葡萄球菌细胞膜上提取下来的膜核糖体保留了一种分子量为 60000 的蛋白质（MBRP），而细胞质核糖体中没有这种蛋白质。通过免疫学方法检测和定量 MBRP。当膜核糖体解离成 50S 和 30S 亚基时，MBRP 仍与 50S 颗粒结合。在膜核糖体和细胞质中发现的 MBRP 数量大致相等。当将抗 MBRP 的抗体添加到 Triton X - 100 溶解的原生质体中时，会产生免疫沉淀，其中包含 MBRP 与另外三种分子量分别为 71000、46000 和 41000 的蛋白质的复合物。发现与膜核糖体相关的四种蛋白质的分子量与 MBRP 复合物中的蛋白质分子量相同。分子量为 71000、60000、46000 和 41000 的蛋白质在复合物中似乎以化学计量相当的量存在。
