Tipperman R, Kasckow J, Herndon R M
J Neuropathol Exp Neurol. 1984 Sep;43(5):522-30. doi: 10.1097/00005072-198409000-00007.
Focal injection of lysolecithin into the thoracic spinal cord was used to induce experimental demyelination in rats. The macrophages which invaded the demyelinating area to phagocytose the myelin breakdown products were examined by electron microscopy using conventional thin-section and freeze-fracture techniques. The cells had the typical foamy cytoplasm characteristic of lipid macrophages. In freeze-fracture preparations, the P-face of the plasmalemma had scattered 10-18 nm round to oval or elongate particles. The cytoplasm was filled with phagocytic vacuoles containing myelin debris. Occasionally, membranous connections could be demonstrated between lysosomes and phagocytic vacuoles. The debris in the vacuoles varied in structure from material with a normal myelin periodicity to partially digested lamellated structures and pseudocrystalline structures to homogeneous-like, lipid-filled vacuoles. These patterns appeared to represent successive stages in the digestion of the ingested myelin.
将溶血卵磷脂局部注入大鼠胸段脊髓以诱导实验性脱髓鞘。采用常规超薄切片和冷冻蚀刻技术，通过电子显微镜检查侵入脱髓鞘区域吞噬髓鞘崩解产物的巨噬细胞。这些细胞具有脂质巨噬细胞典型的泡沫状细胞质特征。在冷冻蚀刻标本中，质膜的P面有散在的10 - 18纳米圆形至椭圆形或细长颗粒。细胞质中充满了含有髓鞘碎片的吞噬泡。偶尔，溶酶体与吞噬泡之间可显示出膜性连接。泡内的碎片结构各异，从具有正常髓鞘周期性的物质到部分消化的层状结构和假晶体结构，再到类似均质的、充满脂质的泡。这些模式似乎代表了摄入髓鞘消化的连续阶段。
