Dissociation of gluconeogenesis from fluid and phosphate reabsorption in isolated rabbit proximal tubules.

Gluconeogenesis in the kidney is a metabolic function characteristic of proximal tubules. Recent studies suggest that renal gluconeogenesis (GNG) may in some way be coupled to fluid and phosphate reabsorption in the proximal tubule. Therefore, the present studies examined more directly the relationship between GNG and transport of fluid and phosphate using isolated proximal straight tubules of rabbit kidney. Glucose production rates were determined in isolated tubules with a glucose microassay while both phosphate (Jp) and net fluid fluxes (Jv) were measured by the in vitro isolated tubule perfusion technique. Glucose production rates from individual substrates, including pyruvate, lactate, glutamate and alpha-ketoglutarate, differed significantly, but neither Jv nor Jp was altered when different substrates in the bath medium were used. Inhibition of GNG by 3-mercaptopicolinate did not alter the Jv or Jp. Acid pH (7.0) stimulated GNG and suppressed both Jv and Jp. The addition of 3-mercaptopicolinate at acid pH abolished the stimulatory effect of acid pH on GNG but had no effect on Jv or Jp. These data thus indicate that, in the isolated rabbit renal proximal tubule, GNG rates can be dissociated from both the Jv and Jp and suggest that renal GNG may not directly regulate the fluid and phosphate transport rates in the proximal straight tubule.