Grdina D J, Linde S, Mason K
Br J Radiol. 1978 Apr;51(604):291-301. doi: 10.1259/0007-1285-51-604-291.
We have characterized selected populations of fibrosarcoma cells separated on density gradients of Renografin with respect to distribution in the cell cycle, clonogenic ability, and response to radiation. The latter two parameters have been observed to be related to the density of the cells, the denser populations being less clonogenic but more resistant to both low and high LET radiation. We have determined that these cells effectively repair potentially lethal damage if allowed to remain in situ following exposure to low LET radiation. This repair ability is not observed, however, following neutron irradiation. In addition, cells left in situ are relatively more sensitive to low doses of either low or high LET radiation. This difference in response is believed to be due to differences in the expression of damage by cells left in situ as compared to those assayed immediately. These data reflect the usefulness of cell separation methods in the study of solid tumour systems. Specifically, these methods reduce the heterogeneity found in the solid tumour by allowing for the isolation of subpopulations which then can be studied either individually or in relation to the entire tumour.
我们已对通过泛影葡胺密度梯度分离出的特定纤维肉瘤细胞群体，在细胞周期分布、克隆形成能力及对辐射的反应方面进行了表征。已观察到后两个参数与细胞密度有关，密度较高的群体克隆形成能力较低，但对低传能线密度和高传能线密度辐射均更具抗性。我们已确定，这些细胞在受到低传能线密度辐射后若留在原位，能够有效修复潜在致死性损伤。然而，中子照射后未观察到这种修复能力。此外，留在原位的细胞对低剂量的低传能线密度或高传能线密度辐射相对更敏感。据信这种反应差异是由于留在原位的细胞与立即检测的细胞相比，损伤表达存在差异。这些数据反映了细胞分离方法在实体瘤系统研究中的实用性。具体而言，这些方法通过分离亚群减少了实体瘤中的异质性，然后可以单独研究这些亚群，或研究它们与整个肿瘤的关系。
