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针吸活检肿瘤细胞与其相应原发肿瘤的密度梯度离心：密度分布比较

Density gradient centrifugation of tumor cells from needle biopsies and their respective source tumors: a comparison of density distributions.

作者信息

Grdina D J, Zin G

出版信息

Experientia. 1983 Aug 15;39(8):916-7. doi: 10.1007/BF01990436.

DOI:10.1007/BF01990436
PMID:6873249
Abstract

A needle biopsy technique was applied to a murine fibrosarcoma (FSa) tumor system. FSa tumors of 8 mm diameter in size were biopsied and then made into single cell suspensions. Resulting density profiles of cells from both sources were compared following centrifugation in Renografin gradients. In all cases, there was excellent agreement between the density profiles of the material from each of the biopsies and the corresponding solid tumors.

摘要

将针吸活检技术应用于小鼠纤维肉瘤（FSa）肿瘤系统。对直径为8毫米的FSa肿瘤进行活检，然后制成单细胞悬液。在泛影葡胺梯度中离心后，比较来自这两种来源的细胞的密度分布。在所有情况下，每次活检材料的密度分布与相应实体瘤的密度分布之间都有很好的一致性。

相似文献

1
Density gradient centrifugation of tumor cells from needle biopsies and their respective source tumors: a comparison of density distributions.针吸活检肿瘤细胞与其相应原发肿瘤的密度梯度离心：密度分布比较
Experientia. 1983 Aug 15;39(8):916-7. doi: 10.1007/BF01990436.
2
Density gradient centrifugation of cells separated from multicellular tumor spheroids.对从多细胞肿瘤球体中分离出的细胞进行密度梯度离心。
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3
Comparison of the densities of clonogenic cells from EMT6 fibrosarcoma monolayer cultures, multicell spheroids, and solid tumors in ficoll density gradients.
J Natl Cancer Inst. 1978 May;60(5):1017-22. doi: 10.1093/jnci/60.5.1017.
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Density centrifugation of murine fibrosarcoma cells following in situ labelling with tritiated thymidine.用氚标记的胸腺嘧啶核苷原位标记后对小鼠纤维肉瘤细胞进行密度离心。
Cell Tissue Kinet. 1981 Nov;14(6):589-600. doi: 10.1111/j.1365-2184.1981.tb00844.x.
5
Studies on a fractionated murine fibrosarcoma: proliferative potential of the separated cells.关于小鼠纤维肉瘤分级分离的研究：分离细胞的增殖潜能
J Cell Physiol. 1979 May;99(2):247-59. doi: 10.1002/jcp.1040990212.
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Separation of cells from a murine fibrosarcoma on the basis of size. I. Relationship between cell size and age as modified by growth in vivo or in vitro.基于大小对小鼠纤维肉瘤细胞进行分离。I. 体内或体外生长对细胞大小与年龄之间关系的影响。
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J Natl Cancer Inst. 1978 Jul;61(1):215-20. doi: 10.1093/jnci/61.1.215.
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Separation of cells from a fibrosarcoma in renografin density gradients.在泛影葡胺密度梯度中从纤维肉瘤分离细胞。
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Local tumour cell seeding by fine-needle aspiration biopsy. A semiquantitative study.细针穿刺活检导致的局部肿瘤细胞种植。一项半定量研究。
Acta Pathol Microbiol Immunol Scand A. 1983 Jan;91(1):17-21. doi: 10.1111/j.1699-0463.1983.tb02721.x.

本文引用的文献

1
Density centrifugation of murine fibrosarcoma cells following in situ labelling with tritiated thymidine.用氚标记的胸腺嘧啶核苷原位标记后对小鼠纤维肉瘤细胞进行密度离心。
Cell Tissue Kinet. 1981 Nov;14(6):589-600. doi: 10.1111/j.1365-2184.1981.tb00844.x.
2
Kinetic heterogeneity in density-separated murine fibrosarcoma subpopulations.密度分离的小鼠纤维肉瘤亚群中的动力学异质性。
Cancer Res. 1982 Dec;42(12):4999-5003.
3
The activation state of macrophage subpopulations from a murine fibrosarcoma.来自小鼠纤维肉瘤的巨噬细胞亚群的激活状态。
Int J Cancer. 1980 Nov 15;26(5):609-15. doi: 10.1002/ijc.2910260513.
4
Radiation response of clonogenic cell populations separated from fibrosarcoma.从纤维肉瘤中分离出的克隆细胞群体的辐射反应。
Radiat Res. 1975 Sep;63(3):483-93.
5
Radiation response of cell populations irradiated in situ and separated from a fibrosarcoma.原位照射并从纤维肉瘤中分离出来的细胞群体的辐射反应。
Radiat Res. 1976 Jun;66(3):634-43.
6
Response of selected tumour cell populations separated from a fibrosarcoma following irradiation in situ with fast neutrons.从原位经快中子照射的纤维肉瘤中分离出的特定肿瘤细胞群体的反应。
Br J Radiol. 1978 Apr;51(604):291-301. doi: 10.1259/0007-1285-51-604-291.
7
Relevance of density, size and DNA content of tumour cells to the lung colony assay.肿瘤细胞的密度、大小和DNA含量与肺集落试验的相关性。
Br J Cancer. 1977 Dec;36(6):659-69. doi: 10.1038/bjc.1977.248.
8
Comparison of the Menghini, Klatskin and Tru-Cut needles in diagnosing cirrhosis.
J Clin Gastroenterol. 1979 Sep;1(3):229-31. doi: 10.1097/00004836-197909000-00007.