儿茶酚胺对大鼠离体比目鱼肌酸碱平衡的短期影响。

The short-term influence of catecholamines on acid-base balance of rat soleus muscle in vitro.

作者信息

Huguenin F

出版信息

Pflugers Arch. 1984 Jul;401(3):272-6. doi: 10.1007/BF00582595.

Abstract

The short-term influence of catecholamines on surface pH (pHs) and intracellular pH (pHi) of superficial muscle fibres has been investigated in rat soleus in vitro using single-barrelled and double-barrelled glass micro-electrodes. pHs means the pH recorded at the surface of a muscle fibre. All measurements were performed in high-Ca2+ (10 mM) Ringer's solutions. Adrenaline caused an intracellular and surface acidification which increased with concentration in the range 6 X 10(-9)-6 X 10(-6) M. The effect was inhibited by propranolol (10(-5)M) but not by phentolamine (1.5 X 10(-7) M). Noradrenaline and isoproterenol (6 X 10(-6) M) also acidified the intracellular fluid. The relative effect of catecholamines on steady-state pHi was: adrenaline = isoproterenol greater than noradrenaline. Adrenaline (6 X 10(-9)-6 X 10(-6) M) did not accelerate pHi recovery following intracellular acid-loading by NH+4 or CO2. It is concluded that activation of beta-adrenoceptors by catecholamines causes an early intracellular acidosis presumably by enhancing synthesis of metabolic acids. The surface acidification seems at least partly due to non-ionic permeation of sarcolemma by metabolic acids, secondarily inducing accumulation of H+ ions at the cell surface.

摘要

利用单管和双管玻璃微电极，在体外对大鼠比目鱼肌进行研究，以探讨儿茶酚胺对浅表肌纤维表面pH值（pHs）和细胞内pH值（pHi）的短期影响。pHs指在肌纤维表面记录的pH值。所有测量均在高钙（10 mM）林格氏液中进行。肾上腺素引起细胞内和表面酸化，在6×10⁻⁹ - 6×10⁻⁶ M范围内，这种酸化随浓度增加而增强。该效应被普萘洛尔（10⁻⁵ M）抑制，但未被酚妥拉明（1.5×10⁻⁷ M）抑制。去甲肾上腺素和异丙肾上腺素（6×10⁻⁶ M）也使细胞内液酸化。儿茶酚胺对稳态pHi的相对效应为：肾上腺素 = 异丙肾上腺素＞去甲肾上腺素。肾上腺素（6×10⁻⁹ - 6×10⁻⁶ M）在通过NH₄⁺或CO₂进行细胞内酸负荷后，并未加速pHi的恢复。得出的结论是，儿茶酚胺激活β - 肾上腺素能受体可能通过增强代谢酸的合成导致早期细胞内酸中毒。表面酸化似乎至少部分是由于代谢酸对肌膜的非离子渗透，继而诱导H⁺离子在细胞表面积累。