大鼠血管活性肠肽的分离与鉴定

Isolation and characterization of rat vasoactive intestinal peptide.

作者信息

Dimaline R, Reeve J R, Shively J E, Hawke D

出版信息

Peptides. 1984 Mar-Apr;5(2):183-7. doi: 10.1016/0196-9781(84)90204-3.

DOI:10.1016/0196-9781(84)90204-3

Abstract

We have used gel filtration, ion exchange chromatography, affinity chromatography and reversed-phase HPLC to isolate vasoactive intestinal peptide from rat intestine. Microsequence analysis of 1 nmole peptide indicated that the sequence was identical to the porcine octacosapeptide VIP. In radioimmunoassay with four antisera and in the turkey pancreas bioassay, rat VIP was equipotent with highly purified preparations of porcine, human and canine VIP. A less basic rat VIP-variant was also isolated and the N-terminal decapeptide region that was sequenced was identical with that of porcine VIP.

摘要

我们已使用凝胶过滤、离子交换色谱、亲和色谱和反相高效液相色谱从大鼠肠道中分离血管活性肠肽。对1纳摩尔该肽进行的微量序列分析表明，其序列与猪的28肽血管活性肠肽相同。在使用四种抗血清进行的放射免疫测定以及火鸡胰腺生物测定中，大鼠血管活性肠肽与高度纯化的猪、人和犬血管活性肠肽制剂具有同等效力。还分离出一种碱性较弱的大鼠血管活性肠肽变体，其测序的N端十肽区域与猪血管活性肠肽的相同。

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