Dimaline R, Thorndyke M C, Young J
Regul Pept. 1986 Mar;14(1):1-10. doi: 10.1016/0167-0115(86)90200-4.
We have used a combination of gel filtration, ion exchange chromatography and reversed-phase HPLC to isolate and characterize a VIP-related peptide from the gut of the elasmobranch Scyliorhinus canicula. The N-terminal decapeptide of the Scyliorhinus material was identical with that of porcine VIP. However, Scyliorhinus VIP did not cross react with antisera specific for the C-terminus of porcine VIP. Like porcine VIP, Scyliorhinus VIP was a potent stimulant of exocrine pancreatic secretion in the turkey, but the response to Scyliorhinus VIP had a shorter duration. VIP from a second elasmobranch, Squalus acanthius was partially purified, and had biological and immunochemical properties similar to those of Scyliorhinus VIP. The results indicate that elasmobranch VIP is identical to porcine VIP at its N-terminus, but differs at the C-terminus. These structural differences may influence the rate of metabolism of the peptide.
我们运用凝胶过滤、离子交换色谱法和反相高效液相色谱法相结合的方法，从板鳃亚纲鱼类小斑猫鲨的肠道中分离并鉴定了一种与血管活性肠肽（VIP）相关的肽。小斑猫鲨提取物的N端十肽与猪VIP的N端十肽相同。然而，小斑猫鲨VIP与针对猪VIP C端的抗血清不发生交叉反应。与猪VIP一样，小斑猫鲨VIP是火鸡外分泌性胰腺分泌的强效刺激物，但对小斑猫鲨VIP的反应持续时间较短。对另一种板鳃亚纲鱼类棘鲨的VIP进行了部分纯化，其生物学和免疫化学特性与小斑猫鲨VIP相似。结果表明，板鳃亚纲鱼类的VIP在N端与猪VIP相同，但在C端不同。这些结构差异可能会影响该肽的代谢速率。
