Farah M O, Lengemann F W, Kogan P, Cetinkaya N
Res Vet Sci. 1984 Jul;37(1):1-6.
Ferritin was isolated from the spleen of sheep and found to contain an iron/protein content of 21 per cent. Electrophoretic behaviour on 6 per cent polyacrylamide gel revealed three protein bands of Rf values of 5.7, 19.3 and 76.1. Sheep ferritin has a molecular weight of about 475,000 daltons and is high in glutamic acid, leucine and aspartic acid and low in methionine and cysteine. Sheep ferritin is not crystallisable by 10 per cent cadmium sulphate and has an ultraviolet-visible spectrum similar to that of bovine ferritin. Anti (sheep) ferritin antibodies raised in rabbits showed a 57.1 per cent binding at a serum dilution of 1:800 and 30 per cent binding at a 1:12,800 dilution, in a titre determination using double antibody assay procedure.
从绵羊脾脏中分离出铁蛋白，发现其铁/蛋白质含量为21%。在6%聚丙烯酰胺凝胶上的电泳行为显示出三条蛋白带，其相对迁移率（Rf）值分别为5.7、19.3和76.1。绵羊铁蛋白的分子量约为475,000道尔顿，谷氨酸、亮氨酸和天冬氨酸含量高，蛋氨酸和半胱氨酸含量低。绵羊铁蛋白不能用10%硫酸镉结晶，其紫外可见光谱与牛铁蛋白相似。用双抗体测定法进行效价测定时，在兔体内产生的抗（绵羊）铁蛋白抗体在血清稀释度为1:800时结合率为57.1%，在1:12,800稀释度时结合率为30%。
