Díez J M, Agapito M T, Recio J M
Rev Esp Fisiol. 1985 Sep;41(3):341-4.
A rapid method of purifying duck liver ferritin using high speed centrifugation and chromatography on Sephadex G-200 and Sepharose 6B is described. Protein and iron concentration for each step of purification is given. This method yields 0.12 mg of pure ferritin per gram of wet tissue.
本文描述了一种利用高速离心以及在葡聚糖凝胶G - 200和琼脂糖6B上进行色谱法快速纯化鸭肝铁蛋白的方法。给出了纯化各步骤的蛋白质和铁浓度。该方法每克湿组织可产生0.12毫克纯铁蛋白。