Bjerknes R
Scand J Haematol. 1984 Aug;33(2):197-206. doi: 10.1111/j.1600-0609.1984.tb02396.x.
The kinetics of human leucocyte phagocytosis and exocytosis of fluorescein-isothiocyanate (FITC)-labelled zymosan particles were studied by flow cytometry (FCM). The leucocytes rapidly associated with zymosan particles, and internalization was confirmed by a fluorescence quenching technique. For incubation periods longer than about 60 min, exocytosis of ingested particles was observed. All human phagocytes ejected zymosan particles. The rate of exocytosis was about 1 particle per phagocyte per h, and was independent of the number of internalized particles. Exocytosis was dependent on temperature and glucose, but did not require Ca++ and Mg++ ions. Phagocytosis and exocytosis occurred concurrently, and phagocytosis accelerated ejection of previously internalized particles. The exocytosed particles were partially degraded by the phagocytes, and rephagocytosis of ejected zymosan particles was slower than the uptake of control particles. Phagocytes undergoing exocytosis remained intact during the 210 min examined. The findings indicate a heretofore neglected vector in the interaction of prey and phagocytosing cells.
通过流式细胞术（FCM）研究了人白细胞对异硫氰酸荧光素（FITC）标记的酵母聚糖颗粒的吞噬作用和胞吐作用动力学。白细胞与酵母聚糖颗粒迅速结合，通过荧光猝灭技术证实了内化过程。对于孵育时间超过约60分钟的情况，观察到摄入颗粒的胞吐作用。所有人类吞噬细胞都排出酵母聚糖颗粒。胞吐速率约为每吞噬细胞每小时1个颗粒，且与内化颗粒的数量无关。胞吐作用依赖于温度和葡萄糖，但不需要Ca++和Mg++离子。吞噬作用和胞吐作用同时发生，吞噬作用加速了先前内化颗粒的排出。胞吐的颗粒被吞噬细胞部分降解，排出的酵母聚糖颗粒的再吞噬比对照颗粒的摄取慢。在检查的210分钟内，经历胞吐作用的吞噬细胞保持完整。这些发现表明在猎物与吞噬细胞相互作用中存在一个迄今被忽视的因素。
