Sako K, Kato A, Diksic M, Yamamoto L Y
Stroke. 1984 Sep-Oct;15(5):896-900. doi: 10.1161/01.str.15.5.896.
We have developed a quantitative autoradiographic technique for the simultaneous measurement of local cerebral blood flow (LCBF) and local cerebral glucose utilization (LCGU) using a combination of 14C-long-lived and 18F-short-lived radionuclides as labels. To obtain the LCGU image, a 50-fold greater radioactivity of 18F than of 14C was administered and the first exposure was done for 2 hours. Three days later, when most of the 18F had decayed, a second exposure was done for 5 to 6 days to obtain the LCBF image. 18F standards were prepared in each experiment. The technique provides, for the first time, the local glucose flow ratio (LGFR). LGFR, obtained by dividing the LCGU by the LCBF image, was expressed as percent mumol/ml (multiplied by 100). Measurement of these three values in the same animal is expected to prove useful in the investigation of the pathophysiology of the brain. The advantages of this method are that cross contamination is less than 4%, chemical or physical manipulation of the slices is unnecessary, and final results can be obtained within a week.
我们已经开发出一种定量放射自显影技术，用于同时测量局部脑血流量（LCBF）和局部脑葡萄糖利用率（LCGU），该技术使用了作为标记的14C长寿命和18F短寿命放射性核素的组合。为了获得LCGU图像，给予的18F放射性比14C高50倍，第一次曝光持续2小时。三天后，当大部分18F衰变后，进行第二次曝光5至6天以获得LCBF图像。每次实验都制备了18F标准品。该技术首次提供了局部葡萄糖流量比（LGFR）。通过将LCGU除以LCBF图像获得的LGFR以每毫升微摩尔百分比表示（乘以100）。预计在同一只动物中测量这三个值将有助于大脑病理生理学的研究。该方法的优点是交叉污染小于4%，无需对切片进行化学或物理操作，并且可以在一周内获得最终结果。
