Child J J, Kurz W G
Can J Microbiol. 1978 Feb;24(2):143-8. doi: 10.1139/m78-026.
Eleven different plant cell tissue cultures of both legume and non-legume origin have been grown in direct association, and in separate but close proximal association with both Spirillum lipoferum and Rhizobium sp. 32H1. Basic similarities were found in the nutritional requirement for the induction of nitrogenase activity (C2H2) in both organisms. In the absence of plant cell cultures both organisms need to be provided with a pentose sugar and a tricarboxylic acid to induce high levels of nitrogen-fixing activity. Plant cell callus tissue appears only capable of supplying the tricarboxylic acid to induce high levels of nitrogen-fixing activity. Plant cell callus tissue appears only capable of supplying the tricarboxylic acids needed but not the sugar component. The plant tissue, however, seems able to activate certain carbohydrates, which in themselves are incapable of substituting for the pentose additive.
已经对11种不同的豆科和非豆科来源的植物细胞组织培养物进行了培养，使其与脂环螺菌和根瘤菌32H1直接联合培养，以及在单独但紧密相邻的条件下培养。在诱导两种生物体中固氮酶活性（C2H2）的营养需求方面发现了基本相似之处。在没有植物细胞培养物的情况下，两种生物体都需要供应戊糖和三羧酸以诱导高水平的固氮活性。植物细胞愈伤组织似乎仅能供应三羧酸以诱导高水平的固氮活性。植物细胞愈伤组织似乎仅能供应所需的三羧酸，而不能供应糖成分。然而，植物组织似乎能够激活某些碳水化合物，而这些碳水化合物本身不能替代戊糖添加剂。
