Kellogg J A
Am J Clin Pathol. 1984 Oct;82(4):455-8. doi: 10.1093/ajcp/82.4.455.
As more broad spectrum antimicrobics continue to appear, many manufacturers have added drugs to their microdilution minimal inhibitory concentration (MIC) test panels at the expense of testing each drug over a broad range of twofold dilutions. Micro Scan (Mahwah, NJ) gram-positive and gram-negative 96-well microdilution panels were tested with the suggested control organisms over a period of 60 days to determine if on-scale MIC endpoints could be achieved with at least one organism per drug. Of 13 antimicrobics in the gram-positive panel, seven (clindamycin, tetracycline, cephalothin, gentamicin, nitrofurantoin, trimethaprim-sulfa, and streptomycin) could not be controlled effectively. Of 18 drugs in the gram-negative panel, six (tobramycin, trimethaprim-sulfa, cefoperazone, mezlocillin, ticarcillin, and kanamycin) similarly could not be controlled. The accuracy of results obtained when patient isolates were tested with these 13 antimicrobics could not be substantiated by control procedures as stated in the Micro Scan instructions.
随着越来越多的广谱抗菌药物不断出现，许多制造商已将药物添加到其微量稀释最低抑菌浓度（MIC）测试板中，却牺牲了在广泛的两倍稀释范围内对每种药物进行测试。对Micro Scan（新泽西州马霍瓦）革兰氏阳性和革兰氏阴性96孔微量稀释板，使用建议的对照微生物进行了为期60天的测试，以确定每种药物是否至少能对一种微生物实现符合标准的MIC终点。在革兰氏阳性测试板的13种抗菌药物中，七种（克林霉素、四环素、头孢噻吩、庆大霉素、呋喃妥因、甲氧苄啶-磺胺和链霉素）无法得到有效控制。在革兰氏阴性测试板的18种药物中，六种（妥布霉素、甲氧苄啶-磺胺、头孢哌酮、美洛西林、替卡西林和卡那霉素）同样无法得到控制。当用这13种抗菌药物对患者分离株进行测试时，所获得结果的准确性无法通过Micro Scan说明书中所述的对照程序得到证实。
