Galgiani J N, Dugger K O, Ito J I, Wieden M A
Am J Trop Med Hyg. 1984 Jul;33(4):645-9. doi: 10.4269/ajtmh.1984.33.645.
Clinical findings in patients with primary infection due to Coccidioides immitis have suggested that fungal antigenemia may occur. To study this possibility, we devised an inhibition enzyme-linked immunosorbent assay (ELISA) to measure antigenic activity compared to the activity of antigens derived from spherules. Twenty-one of 27 sera from 19 infected patients showed antigenic activity above that found in sera from uninfected persons. Activity was highest early in the course of illness. In other studies, anti-spherulin antibody activity was measured by ELISA, which demonstrated an association of antigen activity with IgM, but not IgG or IgA. One possible explanation for this phenomenon is that IgM antibody and antigen are present as circulating immune complexes.
由粗球孢子菌引起的原发性感染患者的临床发现提示可能会发生真菌血症。为研究这种可能性，我们设计了一种抑制酶联免疫吸附测定法（ELISA）来测量抗原活性，并与来自球形体的抗原活性进行比较。19名感染患者的27份血清中有21份显示出高于未感染人群血清中的抗原活性。活性在疾病过程早期最高。在其他研究中，通过ELISA测量抗球孢子菌素抗体活性，结果表明抗原活性与IgM相关，而与IgG或IgA无关。对这一现象的一种可能解释是，IgM抗体和抗原以循环免疫复合物的形式存在。
