Moriyama R, Nakashima H, Makino S, Koga S
Anal Biochem. 1984 Jun;139(2):292-7. doi: 10.1016/0003-2697(84)90005-8.
Alkyl-Sepharose 4B with octyl, decyl, or dodecyl groups as an alkyl chain was a good adsorbent for any type of detergents and a variety of proteins, but not for phospholipids in a vesicle form. When these gels were added to the mixtures of reconstituted proteoliposomes prepared by using bovine band 3 and the protein unincorporated into liposomes, free band 3 in solution was adsorbed onto the gels and the proteoliposomes could be recovered by filtration, suggesting that this procedure, when applicable, permits a rapid isolation of proteoliposomes without loss and dilution of the sample. In addition, the results indicated that Bio-Beads SM-2 resin, which is virtually nonadsorbing for most proteins, can be used in removing any kind of detergents from those protein-detergent mixtures.
带有辛基、癸基或十二烷基链作为烷基链的烷基琼脂糖4B是任何类型洗涤剂和多种蛋白质的良好吸附剂，但对囊泡形式的磷脂不起作用。当将这些凝胶添加到使用牛带3制备的重组蛋白脂质体与未掺入脂质体的蛋白质的混合物中时，溶液中的游离带3会吸附到凝胶上，并且可以通过过滤回收蛋白脂质体，这表明该方法在适用时能够快速分离蛋白脂质体，而不会造成样品损失和稀释。此外，结果表明，对大多数蛋白质几乎无吸附作用的Bio-Beads SM-2树脂可用于从那些蛋白质-洗涤剂混合物中去除任何种类的洗涤剂。
