Cutler A J, Constabel F, Kurz W G, Shargool P D
Anal Biochem. 1984 Jun;139(2):482-6. doi: 10.1016/0003-2697(84)90038-1.
A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed. The method is based on the uptake of liposome encapsulated methylumbelliferyl beta-D-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. Since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease.
已开发出一种用于定量脂质体内容物向长春花原生质体递送的方法。该方法基于对脂质体包裹的甲基伞形酮基β-D-葡萄糖苷的摄取及其酶促水解以产生荧光甲基伞形酮。由于游离葡萄糖苷在很大程度上不会被原生质体摄取，因此可以轻松测量纳摩尔范围内物质的递送情况。
