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用于致癌物检测的肝细胞原代培养DNA修复试验的进一步改进：致癌性联苯衍生物的检测

Further improvements in the hepatocyte primary culture DNA repair test for carcinogens: detection of carcinogenic biphenyl derivatives.

作者信息

Williams G M

出版信息

Cancer Lett. 1978 Feb;4(2):69-75. doi: 10.1016/s0304-3835(78)93522-x.

DOI:10.1016/s0304-3835(78)93522-x
PMID:647653
Abstract

DNA repair in hepatocyte primary cultures was induced by simultaneous treatment with the carcinogen and [3H] thymidine for 18 h beginning immediately after attachment of cells. The unscheduled DNA synthesis elicited by carcinogens was determined by counting grains with an automatic grain counter. In 5 biphenyl derivatives, a correlation was found between carcinogenicity and the ability to induce DNA repair. Hence, the method offers promise as a means of screening chemical carcinogens.

摘要

在肝细胞原代培养物中，从细胞贴壁后立即开始，同时用致癌物和[3H]胸苷处理18小时，诱导DNA修复。通过使用自动颗粒计数器计数颗粒来确定致癌物引发的DNA非常规合成。在5种联苯衍生物中，发现致癌性与诱导DNA修复的能力之间存在相关性。因此，该方法有望成为筛选化学致癌物的一种手段。

相似文献

1
Further improvements in the hepatocyte primary culture DNA repair test for carcinogens: detection of carcinogenic biphenyl derivatives.用于致癌物检测的肝细胞原代培养DNA修复试验的进一步改进：致癌性联苯衍生物的检测
Cancer Lett. 1978 Feb;4(2):69-75. doi: 10.1016/s0304-3835(78)93522-x.
2
Characterization of DNA repair elicited by carcinogens and drugs in the hepatocyte primary culture/DNA repair test.在肝细胞原代培养/DNA修复试验中对致癌物和药物引发的DNA修复进行表征。
J Toxicol Environ Health. 1981 Sep;8(3):463-77. doi: 10.1080/15287398109530083.
3
Induction of unscheduled DNA synthesis in suspensions of rat hepatocytes by an environmental toxicant, 3,3'4,4'-tetrachloroazobenzene.
Cancer Lett. 1979 Apr;6(4-5):207-12. doi: 10.1016/s0304-3835(79)80035-x.
4
Measurement of DNA-excision repair in suspensions of freshly isolated rat hepatocytes after exposure to some carcinogenic compounds: its possible use in carcinogenicity screening.暴露于某些致癌化合物后，对新鲜分离的大鼠肝细胞悬液中DNA切除修复的测量：其在致癌性筛查中的可能用途。
Mutat Res. 1979 Dec;64(6):425-32. doi: 10.1016/0165-1161(79)90112-2.
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Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures.通过大鼠肝脏原代细胞培养中的程序外DNA合成检测化学致癌物。
Cancer Res. 1977 Jun;37(6):1845-51.
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The detection of various nitrosamines in the hepatocyte primary culture/DNA repair test.在肝细胞原代培养/DNA修复试验中对各种亚硝胺的检测。
Cancer Lett. 1979 Apr;6(4-5):199-206. doi: 10.1016/s0304-3835(79)80034-8.
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Use of primary cultures of adult rat hepatocytes on collagen gel-nylon mesh to evaluate carcinogen-induced unscheduled DNA synthesis.利用成年大鼠肝细胞原代培养物在胶原凝胶-尼龙网上评估致癌物诱导的非程序性DNA合成。
Cancer Res. 1980 Sep;40(9):3259-67.
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Carcinogen-induced DNA repair in primary rat liver cell cultures; a possible screen for chemical carcinogens.致癌物诱导的原代大鼠肝细胞培养物中的DNA修复；一种潜在的化学致癌物筛选方法。
Cancer Lett. 1976 Mar;1(4):231-5. doi: 10.1016/s0304-3835(75)97171-2.
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Review of in vitro test systems using DNA damage and repair for screening of chemical carcinogens.利用DNA损伤和修复的体外测试系统筛选化学致癌物的综述。
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Testing of known carcinogens and noncarcinogens for their ability to induce unscheduled DNA synthesis in HeLa cells.检测已知致癌物和非致癌物在HeLa细胞中诱导非定标性DNA合成的能力。
Cancer Res. 1978 Aug;38(8):2621-7.

引用本文的文献

1
Cryopreservation and long-term storage of primary rat hepatocytes: effects on substrate-specific cytochrome P450-dependent activities and unscheduled DNA synthesis.原代大鼠肝细胞的冷冻保存和长期储存：对底物特异性细胞色素P450依赖性活性和非程序性DNA合成的影响。
Cell Biol Toxicol. 1993 Oct-Dec;9(4):345-57. doi: 10.1007/BF00754463.
2
Relationship between the genetically determined acetylator phenotype and DNA damage induced by hydralazine and 2-aminofluorene in cultured rabbit hepatocytes.在培养的兔肝细胞中，遗传决定的乙酰化酶表型与肼屈嗪和2-氨基芴诱导的DNA损伤之间的关系。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1269-72. doi: 10.1073/pnas.79.4.1269.
3
Mutagenicity testing of diethylene glycol monobutyl ether.
二乙二醇单丁醚的致突变性测试。
Environ Health Perspect. 1984 Aug;57:105-12. doi: 10.1289/ehp.8457105.
4
In vitro induction of unscheduled DNA synthesis by genotoxic carcinogens in the hepatocytes of the oyster toadfish (Opsanus tau).
Arch Environ Contam Toxicol. 1985 Sep;14(5):555-63. doi: 10.1007/BF01055385.
5
Quantitative predictivity of carcinogenicity of the autoradiographic repair test (primary hepatocyte cultures) for a group of 80 chemicals belonging to different chemical classes.放射自显影修复试验（原代肝细胞培养）对一组80种属于不同化学类别的化学物质致癌性的定量预测能力。
Environ Health Perspect. 1986 Dec;70:247-53. doi: 10.1289/ehp.8670247.
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Genotoxicity, carcinogenicity, and mode of action of the fried food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ).油炸食品诱变剂2-氨基-3-甲基咪唑[4,5-f]喹啉（IQ）的遗传毒性、致癌性及作用机制
Environ Health Perspect. 1986 Aug;67:121-7. doi: 10.1289/ehp.8667121.
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Use of scintillometric quantitation of unscheduled DNA synthesis in isolated rat hepatocytes for the screening of genotoxic agents.利用闪烁测量法对分离的大鼠肝细胞中DNA的非预定合成进行定量，以筛选遗传毒性剂。
Cell Biol Toxicol. 1987 Jun;3(2):127-42. doi: 10.1007/BF00058452.
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Effect of varying the exposure and 3H-thymidine labeling period upon the outcome of the primary hepatocyte DNA repair assay.改变暴露条件和3H-胸腺嘧啶核苷标记时间对原代肝细胞DNA修复试验结果的影响。
Cell Biol Toxicol. 1988 Jun;4(2):199-209. doi: 10.1007/BF00119246.