Brouns R E, Poot M, de Vrind R, von Hoek-Kon T, Henderson P T, Kuyper C M
Mutat Res. 1979 Dec;64(6):425-32. doi: 10.1016/0165-1161(79)90112-2.
When suspensions of freshly isolated rat hepatocytes were exposed to a number of carcinogenic compounds, it was possible to measure an increased UDS by a rapid procedure via liquid-scintillation counting. For a number of carcinogenic compounds and some of their non-carcinogenic structural analogues a good correlation between the carcinogenic property and the ability to induce UDS was demonstrable. Out of 12 carcinogenic compounds, belonging to several different chemical classes, 10 gave rise to an increased UDS, whereas only 2 compounds, the polycyclic aromatic hydrocarbons benzo[alpha]pyrene and benz[alpha]anthracene, did not. All 4 noncarcinogenic compounds tested were negative. Possibly this method can be of value as a routine screening test, in combination with other short-term test systems, thus improving the predictive value of screening in vitro with respect to carcinogenicity.
当将新鲜分离的大鼠肝细胞悬液暴露于多种致癌化合物时，通过液体闪烁计数的快速程序可以检测到DNA修复合成（UDS）增加。对于多种致癌化合物及其一些非致癌结构类似物，致癌特性与诱导UDS的能力之间存在良好的相关性。在属于几个不同化学类别的12种致癌化合物中，有10种导致UDS增加，而只有2种化合物，即多环芳烃苯并[a]芘和苯并[a]蒽，没有导致UDS增加。所测试的所有4种非致癌化合物均为阴性。该方法可能作为一种常规筛选试验具有价值，与其他短期试验系统相结合，从而提高体外筛选对致癌性的预测价值。
