Hills B A, Butler B D
Ann Rheum Dis. 1984 Aug;43(4):641-8. doi: 10.1136/ard.43.4.641.
Thin-layer chromatography has been used to identify phospholipids extracted from canine synovial fluid, the major component (45%) being phosphatidyl choline (PC). The extracts and their components have been shown to be surface active in reducing the surface tension of water and to be readily adsorbed to hydrophilic solids, whose surfaces then become hydrophobic. These adsorbed monolayers of synovial surfactant were then found to be excellent boundary lubricants in vitro, reducing the coefficient of kinetic friction (mu) in the dry state and under physiological loading by up to 97% for extracts and 99% for PC alone, reaching mu = 0.01. Surface-active phospholipid is put forward as the possible active ingredient in joint lubrication and shown to be consistent with previous biochemical studies to elucidate its identity. The model essentially follows the classical Hardy model for boundary lubrication imparted by surfactants. It is discussed in relation to a new approach in providing artificial lubrication and facilitating tissue release in patients with arthritis.
薄层色谱法已被用于鉴定从犬滑膜液中提取的磷脂，其主要成分（45%）为磷脂酰胆碱（PC）。提取物及其成分已被证明具有表面活性，可降低水的表面张力，并易于吸附到亲水性固体上，这些固体表面随后会变成疏水性。然后发现这些滑膜表面活性剂的吸附单层在体外是极好的边界润滑剂，在干燥状态和生理负荷下，提取物的动摩擦系数（μ）降低高达97%，单独的PC降低高达99%，达到μ = 0.01。表面活性磷脂被认为是关节润滑中可能的活性成分，并被证明与先前阐明其身份的生化研究一致。该模型基本上遵循表面活性剂赋予边界润滑的经典哈迪模型。文中还讨论了一种为关节炎患者提供人工润滑和促进组织松解的新方法。
