Butters T D, Hughes R C
Carbohydr Res. 1978 Mar;61:159-68. doi: 10.1016/s0008-6215(00)84476-6.
High capacity adsorbents for lectins, including Lotus tetragonolobus L-fucose-binding protein, were readily prepared by conjugation of monosaccharides with commercially available, epoxy-activated Sepharose. Purified, radioiodinated lectins were bound to cells of the mosquito Aedes aegyptii and of human KB tumour. Relative to human KB cells, mosquito cells bound less of lectins specific for the sugars (L-fucose and D-galactose) that are terminal residues in many mammalian glycoproteins, whereas the number of binding sites of lectins specific for core-region sugars (D-mannose and 2-acetamido-2-deoxy-D-glucose) were similar. Neuraminidase, which greatly enhanced binding of peanut agglutinin or soybean agglutinin to human KB cells, had negligible effects on binding of these lectins to mosquito cells. The comparative structures of surface oligosaccharides of mosquito and KB cells are discussed in relation to the lectin-binding studies.
通过将单糖与市售的环氧活化琼脂糖偶联，可轻松制备用于凝集素的高容量吸附剂，包括四角豆莲L-岩藻糖结合蛋白。纯化的放射性碘化凝集素与埃及伊蚊和人KB肿瘤细胞结合。相对于人KB细胞，蚊子细胞结合的许多哺乳动物糖蛋白末端残基糖类（L-岩藻糖和D-半乳糖）特异性凝集素较少，而核心区域糖类（D-甘露糖和2-乙酰氨基-2-脱氧-D-葡萄糖）特异性凝集素的结合位点数量相似。神经氨酸酶可大大增强花生凝集素或大豆凝集素与人KB细胞的结合，但对这些凝集素与蚊子细胞的结合影响可忽略不计。结合凝集素结合研究，讨论了蚊子和KB细胞表面寡糖的比较结构。
