Gachelin G, Buc-Caron M H, Lis H, Sharon N
Biochim Biophys Acta. 1976 Jul 15;436(4):825-32. doi: 10.1016/0005-2736(76)90409-0.
We have studied the interaction of five lectins differing in their sugar specificity, with the surface of clonal cell lines derived from transplantable murine teratocarcinoma. The results show that the differentiation from primitive embryonal carcinoma cells into parietal yolk sac cells is accompanied by changes in cell surface saccharides. These changes consist of a marked decrease in the total number of binding sites for the L-fucose-specific lectin of Lotus tetragonolobus and a large increase in the total number of binding sites for wax bean agglutinin. It is suggested that these differences can be used as markers in the study of this early embryonic differentiation. No agglutination of primitive embryonal carcinoma cells or of parietal yolk sac cells by low concentrations (10mug/ml) of concanavalin A, soybean agglutinin or the fucose binding proteins was observed.
我们研究了五种糖特异性不同的凝集素与源自可移植性小鼠畸胎癌的克隆细胞系表面的相互作用。结果表明，从原始胚胎癌细胞分化为壁层卵黄囊细胞的过程伴随着细胞表面糖类的变化。这些变化包括四角豆（Lotus tetragonolobus）的L-岩藻糖特异性凝集素结合位点总数显著减少，以及菜豆凝集素结合位点总数大幅增加。有人提出，这些差异可作为研究这种早期胚胎分化的标志物。未观察到低浓度（10μg/ml）的伴刀豆球蛋白A、大豆凝集素或岩藻糖结合蛋白对原始胚胎癌细胞或壁层卵黄囊细胞的凝集作用。
