碱性胰蛋白酶抑制剂（BPTI）的某些化学修饰对其与特异性抗体反应的影响。
Effect of some chemical modifications of basic pancreatic trypsin inhibitor (BPTI) on its reaction with specific antibody.
作者信息
Prusak E, Kustrzeba-Wójcicka I, Wilusz T, Szewczuk A
出版信息
Arch Immunol Ther Exp (Warsz). 1984;32(1):103-9.
Anti-BPTI-antibody inactivated the antitrypsin activity of basic pancreatic trypsin inhibitor. Esterification of BPTI with methanol did not affect its antitrypsin activity and precipitate formation with antibody. Acetylation, maleylation and hexa-S-carboxylation of BPTI completely inactivated the inhibitor reactivity and markedly diminished its precipitating ability. Performic acid oxidized BPTI and thermolysin digested BPTI lost its antitrypsin as well as antigenic activities. The both preparations as well as oxidized N-acetyl-L-cysteinyl-L-lysyl-L-alanylglycylglycyl-L-cysteine amide did not affect the complex formation between the inhibitor and antibody.
抗BPTI抗体使碱性胰蛋白酶抑制剂的抗胰蛋白酶活性失活。BPTI与甲醇的酯化反应不影响其抗胰蛋白酶活性以及与抗体形成沉淀。BPTI的乙酰化、马来酰化和六-S-羧化完全使抑制剂反应性失活，并显著降低其沉淀能力。过甲酸氧化的BPTI和嗜热菌蛋白酶消化的BPTI失去了抗胰蛋白酶活性以及抗原活性。这两种制剂以及氧化的N-乙酰-L-半胱氨酰-L-赖氨酰-L-丙氨酰甘氨酰甘氨酰-L-半胱氨酸酰胺均不影响抑制剂与抗体之间的复合物形成。
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