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暂时增加离子强度和尿素对DNA与天然及乙酰化BPTI复合物稳定性的影响。

Effect of temporarily increased ionic strength and of urea on stability of DNA complexes with native and acetylated BPTI.

作者信息

Szopa J

出版信息

Arch Immunol Ther Exp (Warsz). 1979;27(1-2):131-40.

PMID:571705
Abstract

Hyperchromism of DNA-BPTI and DNA-acetylated BPTI complexes following dialysis/re-dialysis against solutions of increasing/decreasing concentrations of NaCl, urea or urea and NaCl, amounted to ca 50% of hyperchromism values obtained with free DNA or non-dialyzed complexes Stability of DNA-BPTI and DNA-acetylated BPTI complexes subjected to a temporary effect of NaCl, urea or urea and NaCl resembled stability of the complexes in the presence of Nacl or urea. The presence of all three geometrical DNA forms was detected in re-dialyzed DNA-BPTI and DNA-acetylated BPTI complexes. Possible mechanisms of interaction between BPTI and DNA are discussed.

摘要

将DNA-BPTI和DNA-乙酰化BPTI复合物分别对浓度递增/递减的NaCl、尿素或尿素与NaCl溶液进行透析/再透析后，其增色效应相当于游离DNA或未透析复合物增色效应值的约50%。经受NaCl、尿素或尿素与NaCl暂时作用的DNA-BPTI和DNA-乙酰化BPTI复合物的稳定性，类似于在NaCl或尿素存在下复合物的稳定性。在再透析的DNA-BPTI和DNA-乙酰化BPTI复合物中检测到了所有三种几何形状的DNA形式。文中讨论了BPTI与DNA之间可能的相互作用机制。

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Arch Immunol Ther Exp (Warsz). 1979;27(1-2):131-40.
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