Hołowiecki J, Wojnar J, Stańczuk M, Jarczok K
Arch Immunol Ther Exp (Warsz). 1984;32(1):99-101.
To determine the influence of low temperature (-196 degrees C) during preservation upon the electrophoretic mobility (EM) of lymphocytes ten healthy donors were examined. Lymphocytes were isolated from venous blood by Ficoll-Uropolinum (Polfa) density gradient centrifugation and iron powder technique was used to eliminate macrophages. EM of lymphocytes was analyzed on Parmoquant 2 computer system (Carl-Zeiss, Jena) before and ten days after cryopreservation. There was no significant difference between the mean EM before freezing (1.046 um.sec-1.v-1.cm) and after it (1.034 um.sec.-1v-1 cm). No significant changes in histograms showing slow (lymphocytes B) and fast (lymphocytes T) fractions were observed. It indicates, that cryopreservation does not change EM of lymphocytes and thus can be used in situations, when immediate examination cannot be done.
为了确定保存期间的低温（-196℃）对淋巴细胞电泳迁移率（EM）的影响，对10名健康供者进行了检查。通过Ficoll-尿嘧啶（Polfa）密度梯度离心从静脉血中分离淋巴细胞，并采用铁粉技术去除巨噬细胞。在冷冻保存前和冷冻保存十天后，使用Parmoquant 2计算机系统（卡尔蔡司，耶拿）分析淋巴细胞的EM。冷冻前的平均EM（1.046μm·sec⁻¹·v⁻¹·cm）与冷冻后的平均EM（1.034μm·sec⁻¹·v⁻¹·cm）之间无显著差异。在显示缓慢（B淋巴细胞）和快速（T淋巴细胞）组分的直方图中未观察到显著变化。这表明，冷冻保存不会改变淋巴细胞的EM，因此可用于无法立即进行检查的情况。
