一种简单、快速且高效的红细胞铜锌超氧化物歧化酶分离方法。
A simple, rapid and efficient isolation of erythrocyte Cu2Zn2-superoxide dismutase.
作者信息
Gärtner A, Hartmann H J, Weser U
出版信息
Biochem J. 1984 Jul 15;221(2):549-51. doi: 10.1042/bj2210549.
Abstract
On the basis of the thermal stability of erythrocuprein (Cu2Zn2-superoxide dismutase) a rapid preparation technique was devised and successfully employed to isolate this protein. Partial heat-deterioration of the haemolysate and subsequent chromatography of the supernatant on DEAE-Sephacel and Sephadex G-75 yielded an electrophoretically homogeneous protein within a few days. The physicochemical properties and biochemical function were identical with those reported for Cu2Zn2-superoxide dismutases prepared by established methods.
摘要
基于红细胞铜蛋白（Cu2Zn2 - 超氧化物歧化酶）的热稳定性，设计并成功采用了一种快速制备技术来分离这种蛋白质。对溶血产物进行部分热降解，然后将上清液在DEAE - 葡聚糖凝胶和葡聚糖凝胶G - 75上进行层析，在几天内得到了电泳纯的蛋白质。其物理化学性质和生化功能与通过既定方法制备的Cu2Zn2 - 超氧化物歧化酶所报道的性质相同。
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本文引用的文献
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Erythrocuprein (Cu2Zn2 superoxide dismutase) is the major copper protein of the red blood cell.红细胞铜蛋白（Cu2Zn2超氧化物歧化酶）是红细胞的主要铜蛋白。
FEBS Lett. 1983 May 2;155(1):15-8. doi: 10.1016/0014-5793(83)80199-9.
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Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
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A study on purified bovine erythrocuprein.一项关于纯化牛红细胞铜蛋白的研究。
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Inhibition of nitroblue tetrazolium reduction by cuprein (superoxide dismutase), Cu(tyr)2 and Cu(lys)2.铜蛋白（超氧化物歧化酶）、Cu(tyr)2和Cu(lys)2对氮蓝四唑还原的抑制作用。
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