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利用氢核磁共振波谱法对人红细胞超氧化物歧化酶进行研究。

Investigation of human erythrocyte superoxide dismutase by 1H nuclear-magnetic-resonance spectroscopy.

作者信息

Hill H A, Lee W K, Bannister J V, Bannister W H

出版信息

Biochem J. 1980 Jan 1;185(1):245-52. doi: 10.1042/bj1850245.

DOI:10.1042/bj1850245
PMID:7378050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161291/
Abstract

The 170MHZ 1 H n.m.r. spectra of the Cu(II)/Zn(II), Cu(I)/Zn(II) and apo- forms of human erythrocyte superoxide dismutase (EC 1.15.1.1) are reported. Resonances are assigned to the C-2 and C-4 protons of histidine residues in the active site, and it is suggested that five or six histidine residues serve as ligands to the metal ions in each subunit of the enzyme. The remaining assigned resonances are associated with histidine-41, N-terminal N-acetyl group, histidine- 108 and cysteine- 109. A comparison of the n.m.r. spectra of human and bovine superoxide dismutases suggests significant structural homology.

摘要

报道了人红细胞超氧化物歧化酶（EC 1.15.1.1）的铜（II）/锌（II）、铜（I）/锌（II）和脱辅基形式的170兆赫质子核磁共振谱。共振峰归属于活性位点中组氨酸残基的C-2和C-4质子，并且表明在酶的每个亚基中有五个或六个组氨酸残基作为金属离子的配体。其余已归属的共振峰与组氨酸-41、N端N-乙酰基、组氨酸-108和半胱氨酸-109有关。人和牛超氧化物歧化酶的核磁共振谱比较表明存在显著的结构同源性。

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本文引用的文献

1
Preparation of pure bovine apo-erythrocuprein by gel filtration.通过凝胶过滤法制备纯牛脱辅基红细胞铜蛋白。
FEBS Lett. 1971 Sep 15;17(1):78-80. doi: 10.1016/0014-5793(71)80567-7.
2
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
J Biol Chem. 1969 Nov 25;244(22):6049-55.
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Nuclear magnetic resonance spectra of human and bovine superoxide dismutases.人和牛超氧化物歧化酶的核磁共振光谱
FEBS Lett. 1973 May 15;32(1):119-23. doi: 10.1016/0014-5793(73)80752-5.
4
Studies on the reconstitution of bovine erythrocyte superoxide dismutase. IV. Preparation and some properties of the enzyme in which Co(II) is substituted for Zn(II).牛红细胞超氧化物歧化酶的重组研究。IV. 用Co(II)取代Zn(II)的酶的制备及某些性质
J Biol Chem. 1973 Jun 25;248(12):4229-34.
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Bovine erythrocyte superoxide dismutase. Complete amino acid sequence.牛红细胞超氧化物歧化酶。完整氨基酸序列。
J Biol Chem. 1974 Nov 25;249(22):7326-38.
6
Pulse methods for the simplification of protein NMR spectra.用于简化蛋白质核磁共振谱的脉冲方法。
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Alpha-carbon coordinates for bovine Cu,Zn superoxide dismutase.牛铜锌超氧化物歧化酶的α-碳原子坐标。
Biochem Biophys Res Commun. 1975 Apr 21;63(4):986-92. doi: 10.1016/0006-291x(75)90666-x.
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Crystal structure of bovine Cu,Zn superoxide dismutase at 3 A resolution: chain tracing and metal ligands.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1349-53. doi: 10.1073/pnas.72.4.1349.
9
Carbon-2 proton exchange at histidine-41 in bovine erythrocyte superoxide dismutase.牛红细胞超氧化物歧化酶中组氨酸-41处的碳-2质子交换
Biochem J. 1977 Sep 1;165(3):587-9. doi: 10.1042/bj1650587.
10
Investigation of the structure of bovine erythrocyte superoxide dismutase by 1H nuclear magnetic resonance spectroscopy.利用核磁共振氢谱对牛红细胞超氧化物歧化酶结构的研究。
Biochemistry. 1977 Jul 12;16(14):3061-6. doi: 10.1021/bi00633a003.

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