Dresel H A, Otto I, Weigel H, Schettler G, Via D P
Biochim Biophys Acta. 1984 Oct 4;795(3):452-7. doi: 10.1016/0005-2760(84)90172-3.
Triton X-114 was used to solubilize the membrane proteins of bovine adrenal cortex and human leukocytes. The solubilized membrane proteins were subjected to electrophoresis and transblotted to nitrocellulose paper and incubated with LDL/acetyl-LDL. The combination of peroxidase-conjugated second antibody and 4-chloronaphthol/H2O2 allowed rapid development of colored bands where LDL or acetyl-LDL bound to electroblotted proteins. The ELISA is highly sensitive and efficient for screening a large number of samples and avoids the need for a continuous supply of radiolabeled antibodies and autoradiography.
用 Triton X - 114 溶解牛肾上腺皮质和人白细胞的膜蛋白。将溶解的膜蛋白进行电泳并转印到硝酸纤维素纸上，然后与低密度脂蛋白/乙酰化低密度脂蛋白一起孵育。过氧化物酶偶联的二抗与 4 - 氯萘酚/H₂O₂ 的组合使得低密度脂蛋白或乙酰化低密度脂蛋白结合到电转印蛋白的部位能快速显色。酶联免疫吸附测定（ELISA）对于筛选大量样品具有高度敏感性和高效性，并且无需持续供应放射性标记抗体和进行放射自显影。
