外在标签在细菌视紫红质一级和三级结构中的位置。
Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin.
作者信息
Katre N V, Finer-Moore J, Stroud R M, Hayward S B
出版信息
Biophys J. 1984 Aug;46(2):195-203. doi: 10.1016/S0006-3495(84)84013-8.
Abstract
We located a heavy metal label, mercurilated phenylglyoxal, in both the primary sequence and in the tertiary structure of bacteriorhodopsin. This label modified arginines 225 and 227, which are on the COOH-terminal helix (G). In the projected electron potential difference map, the major site is close to the central inner helix. From this result we conclude that helix 1 could not be the COOH-terminal helix G. We tested the multiple isomorphous replacement method for obtaining phases for purple membrane by electron diffraction.
摘要
我们在细菌视紫红质的一级序列和三级结构中都找到了一种重金属标记物，即汞化苯乙二醛。该标记物修饰了位于COOH末端螺旋（G）上的精氨酸225和227。在预测的电子电位差图中，主要位点靠近中央内部螺旋。根据这一结果，我们得出结论，螺旋1不可能是COOH末端螺旋G。我们测试了通过电子衍射获得紫膜相位的多同晶置换法。
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