Fossier P, Baux G, Tauc L
Brain Res. 1984 Aug 13;308(2):369-72. doi: 10.1016/0006-8993(84)91080-1.
At Aplysia H- and D-type cholinergic neuro-neuronal synapses, application of high concentrations of detergents (Triton X-100 and sodium deoxycholate) depressed synaptic transmission and the postsynaptic response to ionophoretic application of acetylcholine (ACh) or carbachol. However, when very low concentrations of detergents (of the order of 10(-9) M for sodium deoxycholate) were used, the nerve-evoked response as well as the ACh and carbachol ionophoretic responses were facilitated (by at least 200%), but only in H-type cells. This facilitation was similar to that previously observed in the same receptor type when acetylcholinesterase (AChE) was inhibited by various organophosphate or carbamate acetylcholinesterase inhibitors (AChEIs)3. Indeed, the effects of AChEI and detergents were not cumulative. We propose that on H-type synapses detergents may perturb a hypothetical molecular interaction between AChE and the acetylcholine receptor (AChR) by which AChE modulates the ability of the AChR to be activated by ACh or carbachol.
在海兔的H型和D型胆碱能神经-神经元突触中，应用高浓度去污剂（曲拉通X-100和脱氧胆酸钠）会抑制突触传递以及对离子电泳施加乙酰胆碱（ACh）或卡巴胆碱的突触后反应。然而，当使用极低浓度的去污剂（脱氧胆酸钠约为10^(-9) M）时，神经诱发反应以及ACh和卡巴胆碱离子电泳反应会增强（至少增强200%），但仅在H型细胞中如此。这种增强类似于之前在同一受体类型中当乙酰胆碱酯酶（AChE）被各种有机磷酸酯或氨基甲酸酯类乙酰胆碱酯酶抑制剂（AChEIs）抑制时所观察到的情况。实际上，AChEI和去污剂的作用并非累加的。我们提出，在H型突触上，去污剂可能会扰乱AChE与乙酰胆碱受体（AChR）之间一种假设的分子相互作用，通过这种相互作用，AChE调节AChR被ACh或卡巴胆碱激活的能力。
