Baux G, Tauc L
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5126-8. doi: 10.1073/pnas.80.16.5126.
Carbachol was injected into a presynaptic cholinergic neuron in the buccal ganglion of Aplysia and the quantal aspects of the Cl- -dependent postsynaptic response to a prolonged stimulation were analyzed by a statistical fluctuation method. The calculated amplitude of the miniature postsynaptic current was increased with respect to control. Statistical fluctuation analysis was also used to analyze the postsynaptic response obtained during ionophoretic application of acetylcholine and carbachol. The calculated unitary channel current was found to be greater for carbachol than for acetylcholine. This increase could explain the larger miniature postsynaptic current seen after intracellular injection of carbachol into the presynaptic neuron if carbachol was released at the synapse as a false transmitter. This conclusion was supported by the observation that it was possible to restore transmission at a synapse previously blocked by presynaptic intracellular injection of acetylcholinesterase with a presynaptic injection of carbachol.
将氨甲酰胆碱注入海兔颊神经节的突触前胆碱能神经元,并用统计涨落方法分析了对长时间刺激的氯离子依赖性突触后反应的量子方面。计算得到的微小突触后电流幅度相对于对照有所增加。统计涨落分析还用于分析离子电泳施加乙酰胆碱和氨甲酰胆碱时获得的突触后反应。发现氨甲酰胆碱的计算单位通道电流大于乙酰胆碱。如果氨甲酰胆碱作为假递质在突触处释放,这种增加可以解释在突触前神经元内注入氨甲酰胆碱后所见的较大微小突触后电流。这一结论得到以下观察结果的支持:通过突触前注入氨甲酰胆碱,可以恢复先前因突触前细胞内注入乙酰胆碱酯酶而阻断的突触处的传递。