从用于冲洗超排母牛子宫和输卵管的液体中回收的淋巴细胞中分离牛合胞体病毒。
Isolation of bovine syncytial virus from lymphocytes recovered from fluids used to flush uterus and oviducts of superovulated cattle.
作者信息
Bouillant A M, Ruckerbauer G M
出版信息
Can J Comp Med. 1984 Jul;48(3):332-4.
Abstract
A Ficoll-gradient method was applied to isolate lymphocytes from fluids used to flush the uterus and oviducts of superovulated cows. Bovine syncytial virus antigens were demonstrated in 15 of 19 cows by cocultivation of lymphocytes with fetal lamb spleen cells and examining them with direct immunofluorescence. Viral serum antibodies were found in the same 15 of 19 cows as above by the modified direct complement-fixation test. The virus was also detected in one of four uterotubal cell cultures.
摘要
采用Ficoll梯度法从用于冲洗超排奶牛子宫和输卵管的液体中分离淋巴细胞。通过将淋巴细胞与胎羊脾细胞共培养并用直接免疫荧光法检测，在19头奶牛中的15头检测到牛合胞体病毒抗原。通过改良直接补体结合试验，在上述19头奶牛中的同样15头中发现了病毒血清抗体。在4份子宫输卵管细胞培养物中的1份中也检测到了该病毒。
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