Gómez M I, Santolaya R C, Cabada M O
Cell Tissue Res. 1984;237(1):191-4. doi: 10.1007/BF00229217.
Observation of the cortical region of oocytes of Bufo arenarum by transmission electron microscopy reveals modifications on their surface and in the contents of the cortical granules (CG) during activation. In non-activated oocytes only amorphous cortical granules (ACG) can be observed. Activated oocytes display ACG, intermediate cortical granules containing both amorphous and membranous material (ICG), and a third type containing only membranous material (MCG). During exocytosis, CG release their contents into the perivitelline space, where the amorphous and membranous materials are found. The three types of CG found during oocyte activation suggest transformation of ACG to MCG and indicate that the different components of the cortical granules, when released into the perivitelline space, might play different roles in prevention of polyspermy.
通过透射电子显微镜观察阿根廷蟾蜍卵母细胞的皮质区域，发现激活过程中其表面和皮质颗粒（CG）内容物存在变化。在未激活的卵母细胞中，只能观察到无定形皮质颗粒（ACG）。激活的卵母细胞显示出ACG、含有无定形和膜状物质的中间皮质颗粒（ICG）以及仅含有膜状物质的第三种类型（MCG）。在胞吐过程中，CG将其内容物释放到卵周隙中，在那里发现了无定形和膜状物质。卵母细胞激活过程中发现的三种类型的CG表明ACG向MCG的转变，并表明皮质颗粒的不同成分释放到卵周隙中时，可能在防止多精受精中发挥不同作用。
