Haemmerli G, Arnold B, Sträuli P
Cell Biol Int Rep. 1984 Aug;8(8):689-702. doi: 10.1016/0309-1651(84)90049-3.
Most of the studies dealing with cellular shape, surface configuration, and motility are carried out in vitro on plane substrata. During the past years, the direct transfer of results obtained under these conditions to the cellular behavior displayed in the living organism, has been increasingly challenged. For this reason we have investigated the above mentioned functions of different cell classes localized on glass and in the loose connective tissue. The cells utilized were: fibroblasts and macrophages from normal rat and rabbit mesenteries, V2 rabbit carcinoma cells and L5222 rat leukemia cells. The combination of time-lapse cinematography and scanning electron microscopy revealed that motility and surface features are the same, irrespective of the immediate surrounding. Cellular shape and attachment, on the other hand, are dependent on the substrate. Fibroblasts, macrophages and cells of epithelial origin, including carcinoma cells, flatten on glass, but have a rounded configuration in the tissue. The flat leading lamellae displayed during locomotion on glass, are not evident in cells migrating through tissues. What regards attachment devices, extensively studied on glass, their formation and position within a tissue are, at present, a matter of speculation. Although it can be assumed that a similar process is operable in vivo and in vitro, clarification rests upon the use of ultrahistochemical techniques.
大多数关于细胞形状、表面形态和运动性的研究是在体外平面基质上进行的。在过去几年中，将在这些条件下获得的结果直接应用于活生物体中细胞行为的做法，受到了越来越多的质疑。因此，我们研究了位于玻璃和疏松结缔组织中的不同细胞类型的上述功能。所使用的细胞有：正常大鼠和兔肠系膜中的成纤维细胞和巨噬细胞、V2兔癌细胞和L5222大鼠白血病细胞。延时摄影和扫描电子显微镜的结合显示，无论周围环境如何，细胞的运动性和表面特征都是相同的。另一方面，细胞形状和附着取决于基质。成纤维细胞、巨噬细胞和上皮来源的细胞，包括癌细胞，在玻璃上会变平，但在组织中呈圆形。在玻璃上运动时显示的扁平前缘薄片，在穿过组织迁移的细胞中并不明显。关于在玻璃上广泛研究的附着装置，它们在组织中的形成和位置目前仍是推测的问题。虽然可以假定在体内和体外存在类似的过程，但仍需使用超组织化学技术来加以阐明。
